Sequences from confirmation amplicon


Confirmation sequences for line 872F11 and hit At1g79620 are shown.
Line ID 872F11
Locus At1g79620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29960763
Primers used for PCR EU87 - 8474, show primer details
   
Sequence Name 39-K103934w-0022-872-F11C-EU87
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, EU87: 5'-TTTTCAGTATTCTTGCTGGTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103934w-0022-872-F11C-EU87
CTCAAGATCTATCTTTCTTGTAGAAAATAGCATTCCGTAGTGTTTGATATTGTCCTCAAA
CCAACTCATGTTTGATGAGACTTGCCACTTTGGTTCCACCATGTTTTCTCAGGGCCTTGA
ACTCGAACAACTTCACGGGTAAAATCCCGGCGTCTCTAGGAAATCTCACTAAGGTTTATT
GGCTGGATCTTGCGGATAATCAGTTGACAGGACCCATTCCAATTTCATCAGGCTCAAGTC
CTGGTCTTGATCTTCTTTTGAAAGCCAAACACTTGTGAGTAATTTTATTTTCTCTGCAGA
CATCTTCTTCAGCTTCAAGTACCTTTCTTCTGATGCAAATGTTATAAAAACTGCAGTCAT
TTCAACAAGAATCAGCTCTCTGGGACCATTCCACCAAAACTTTTCAGCTCTGAGATGATA
CTGATCCATGTGTAAGTCAAATACACTTCTAATAATATTACATATTTGTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTA
   
Sequence Name 50-K103935w-0022-872-F11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K103935w-0022-872-F11C-8409
TCCGGACTGAGCCTTTACATTGAATATATCCTGACAATATGTAATATTATTACCAAGTGT
ATTTGACTTACACATGGATCAGTATCATCTCAGAGCTGAAAAGTTTTGGTGGAATGGTCC
CAGAGAGCTGATTCTTGTTGAAATGACTGCAGTTTTTATAACATTTGCATCAGAAGAAAG
GTACTTGAAGCTGAAGAAGATGTCTGCAGAGAAAATAAAATTACTCACAAGTGTTTGGCT
TTCAAAAGAAGATCAAGACCAGGACTTGAGCCTGATGAAATTGGAATGGGTCCTGTCAAC
TGATTATCCGCAAGATCCAGCCAATAAACCTTAGTGAGATTTCCTAGAGACGCCGGGATT
TTACCCGTGAAGTTGTTCGAGTTCAAGGCCCTGAGAAAACATGGTGGAACCAAAGTGGCA
AGTCTCATCAAACATGAGTTGGTTTGAGGACAATATCAAACACTGCAAAATGCTATTTTC
TTACAAGAAAGATAGATCTTTGAGATAGCCAAGCTCATTTGGGATGGTACCAGTGAAGCC
ACAACCAGCAAGAATACTGAAAA
Primers used for PCR CN32 - 3144, show primer details
   
Sequence Name 68-K106975-0022-872-F11C-3144
GenBank Accession LN512026 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K106975-0022-872-F11C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGGGTACCCGGGGATCCA
TGTGTAGGTTCTGTGAGTAACATATCATCACTATGTCCTTAGTTTCATGTATCTTCCCTA
TCTTCAAAAAGATATAACTCTTGTATTTGCAACAGTCGGCTTGACAGAAACACTTTGACC
GGAAAAGTTCCAGAGAATCTCAGTAATCTCACAAACATCATTGAACTGTAAGTGTTGCCA
GAGTTATGATATTACTATATAGATCATATTAGACATCACTTATGAATGTATACATCTTTT
TTTCTGTCTCTCAGGAACTTAGCTCACAACAAGCTCGTTGGATCATTACCAGATTTGTCA
GACATGAAGTCCATGAACTACGTGTAAATTTCTCACCAACAGAGAACGTCTTTATCTACA
TTTATGCGTTTGTGTTCTCTGATTAAATGTGATGTCTGTTACAGAGATCTCAGCAATA
   
Sequence Name 84-K106977-0022-872-F11C-CN32
GenBank Accession LN514435 [GenBank]
Sequencing primer gene specific primer, CN32: 5'-TGGATCAAATGAGTTATTGCTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K106977-0022-872-F11C-CN32
ATTTATCAGAGACACAAACGCATAAATGTAGATAAGACGTTCTCTGTTGGTGAGAAATTT
ACACGTAGTTCATGGACTTCATGTCTGACAAATCTGGTAATGATCCAACGAGCTTGTTGT
GAGCTAAGTTCCTGAGAGACAGAAAAAAAGATGTATACATTCATAAGTGATGTCTAATAT
GATCTATATAGTAATATCATAACTCTGGCAACACTTACAGTTCAATGATGTTTGTGAGAT
TACTGAGATTCTCTGGAACTTTTCCGGTCAAAGTGTTTCTGTCAAGCCGACTGTTGCAAA
TACAAGAGTTATATCTTTTTGAAGATAGGGAAGATACATGAAACTAAGGACATAGTGATG
ATATGTTACTCACAGAACCTACACATGGATCCCCGGGTACCCTGATAGTTTAAACCGAAG
GCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCT
TATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTG
GAGATATCA

Last Updated on 06.12.2018 16:11