Sequences from confirmation amplicon


Confirmation sequences for line 873D02 and hit At4g38220 are shown.
Line ID 873D02
Locus At4g38220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17925523
Primers used for PCR 12ed - 8474, show primer details
   
Sequence Name 79-K110680-0022-873-D02C-12ed
GenBank Accession LN513840 [GenBank]
Sequencing primer gene specific primer, 12ed: 5'-AATCTGCGAATACTCTCAATGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K110680-0022-873-D02C-12ed
AGCTTGGCACCGTGGCCAGGTGGACCTTTAGCCTTAATCACCAGCCACCAGGGACTCCTC
TCTCCATAGAATACTCTGTAACTCTCAGTAGGCGATGGCAGGCCTGATTCACTTTGTTGT
ATAAGATGATGCTTGGCTGTTATAATCAAGAATCAAATCCAGAAGAGTGTTTACCTTCGT
CGAGCACGATTGCGATGTTCAAGCTCTTGAATAATTGGGATTCAGCAAACTTCTCTGCGC
CATCGTGGCCGCCAATCTCTTCATCGGGGACGAAGGAGAGATAGACGGATCGGAGTGGCT
TGAAGCCAGAAGCCTGGAGCTTGCGTATGGCCTCGAGGTACTGCATCCCGACGCACTTCA
TGTCCTGGGAACCCCTGGCATAGATGTCGCCATGGTGGTCCATGTGAGCTTGGAGCGGAT
GGTGAGTCCACTTGGAGTCCTCGAAGGGAACGACATCGGTGTGGGAGTTGAGGAGAAAGG
CAGGTAGGGTTGGGTCGGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 71-K110679-0022-873-D02C-8409
GenBank Accession LN512579 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K110679-0022-873-D02C-8409
CTCACTCCACACCGATGTCGTTCCCTTCGAGGACTCCAAGTGGACTCACCATCCGCTCCA
AGCTCACATGGACCACCATGGCGACATCTATGCCAGGGGTTCCCAGGACATGAAGTGCGT
CGGGATGCAGTACCTCGAGGCCATACGCAAGCTCCAGGCTTCTGGCTTCAAGCCACTCCG
ATCCGTCTATCTCTCCTTCGTCCCCGATGAAGAGATTGGCGGCCACGATGGCGCAGAGAA
GTTTGCTGAATCCCAATTATTCAAGAGCTTGAACATCGCAATCGTGCTCGACGAAGGTAA
ACACTCTTCTGGATTTGATTCTTGATTATAACAGCCAAGCATCATCTTATACAACAAAGT
GAATCAGGCCTGCCATCGCCTACTGAGAGTTACAGAGTATTCTATGGAGAGAGGAGTCCC
TGGTGGCTGGTGATTAAGGCTAAAGGTCCACCTGGCCACGGTGCCAAGCTCTATGACAAC
TCTGCCATGGAGAATCTGCTCAAAAGCATTGAGAGTATTCGCAGAT

Last Updated on 06.12.2018 16:11