Sequences from confirmation amplicon


Confirmation sequences for line 877C08 and hit At4g26100 are shown.
Line ID 877C08
Locus At4g26100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13230354
Primers used for PCR KQ84 - 8474, show primer details
   
Sequence Name 27-K101500-0022-877-C08C-8409
GenBank Accession LN498318 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K101500-0022-877-C08C-8409
CGGAATGAGCCATTTACAATTCGATAATATTAACAACAATGTTAGAGATAATGTGACGAA
TTCGATAATCCATAAAATTAAAAACTAGAAAGTGAGAGAGAGACGAACCGAGATAGATCT
CTCCAAACGAACCACTACCGATCTTTCGGCCGAGACGAAACTTGTTCCCCACACGAGGTT
CCATAAATAACCACGAAACCCCTTGTACAATCTTAACAACCCAGACGCAGATTCAAATAG
AGCGAAAGGGACCCCAAAAACAATCCTGACTAGAATCAGCCGCACCAGCAATCCAGAAAA
ACAACCCAGAGATCACGAACAATGAGATTACGGATTTTTCGCCGACTAAATCAAAACCCA
GTAATCATCTTCTTCGTGCTCTCGATCTGGTGAAAAAACTCCATATGAACAGAATGTCGT
TTTCTGGGATGCTTCTCTCTCTCTCTCTCCTCTCAAGAATTGAATACGAAGATNAAAAAA
AAAAAAAAAAAAAAAACTTTCCGTGGAAAAAAAACAATAAGGATGTCTTTCCCAAAGTTG
GCGCCTTTTTTTTTTCCCCCTTCCCGGGGAGTGGGGGGAAAGGAAAAAAAATTTTTCCC
   
Sequence Name 27-K101504-0022-877-C08C-KQ84
GenBank Accession LN498319 [GenBank]
Sequencing primer gene specific primer, KQ84: 5'-CCGGAATCATTCAGAGGTAAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K101504-0022-877-C08C-KQ84
GAGGAAAAAAAAAGACGCAAACTTTGAGAAAGACATCCTCATCGTTTTCTGTCCATCGAA
GCTCTTTTTTTTTTTTTTTTTTTTAACCCCCGAATCCATTCCTGGGGGGGAAAAAAAAAA
AAAAACCCCCCCCAAAAAACAACTTTTTGTCCAAGGGGGGTTTTTCCCCCAAACGGGGGC
CCCAAAAAAAAAAATCCGGGGGTTTTATTTTACCCGGAAAAAAACCCAAACCCCCTTGGG
CGGGAACCCGGGGGTGGTTTTCCGGGAGGGGGGGGGGGGGGAATTCTTGCAGGGATGGTT
TTGGGGGCCCCTTCCCCCCTTTTAAAACCCCCCCGGGGGGGGAAAAAATGGAAAAGGGGG
TTCCGGGGTTTTTTAGGAACCCCCGGGGGGGAAAAAATTTCCCCCCCCCCCAAAAAACCG
GTGGGGGTCCTTTGGAAAAAATTTTTTGGGGCCCCCCCCTCTCCCAATTTCGGGTTTTAA
ATTTTGGGGAAAACAAAACCCCCCCAATTTCCCCAAAAATGGTTGTAAAAATAACAAAAT
GGAAAGGGGCCCCCGGCCCGGGAAACCCCCGG

Last Updated on 06.12.2018 16:11