Sequences from confirmation amplicon


Confirmation sequences for line 878C04 and hit At1g62870 are shown.
Line ID 878C04
Locus At1g62870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23286284
Primers used for PCR 11sv - 8474, show primer details
   
Sequence Name 42-K116654-0022-878-C04C-11sv
GenBank Accession LN504605 [GenBank]
Sequencing primer gene specific primer, 11sv: 5'-CTTAATTTCGTTCAGGGTTGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116654-0022-878-C04C-11sv
CAACGCACAACACGGCGACACAACAGCAAACGCAAACGCAATCACCAACGCAACAACCGT
CGGCGGAAGAATTAGCGACAAAGGCGTTACAAAAGCGTTACGAAGGATTAATGATGGTAA
GAACAAAAGCAGTTAAAGGCAAAGGCGCGTGGTATTGGAGTCACCTTGAACCAATCTTAC
TACACAACACCGACACTGGTTTCCCTAAACCTACATGGATCAATTGTAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 17-K116653-0022-878-C04C-8409
GenBank Accession LN494020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116653-0022-878-C04C-8409
TTGAATATATCCTACAATTGATCCATGTAGGTTTAGGGAAACCAGTGTCGGTGTTGTGTA
GTAAGATTGGTTCAAGGTGACTCCAATACCACGCGCCTTTGCCTTTAACTGCTTTTGTTC
TTACCATCATTAATCCTTCGTAACGCTTTTGTAACGCCTTTGTCGCTAATTCTTCCGCCG
ACGGTTGTTGCGTTGGTGATTGCGTTTGCGTTTGCTGTTGTGTCGCCGTTGTTGTTGCGT
TTGTCGTCGTCGCCATTGTAAAGTCAAAAAACTTAAACAACAACCCTGAACGAAATTAA

Last Updated on 06.12.2018 16:11