Sequences from confirmation amplicon


Confirmation sequences for line 878D10 and hit At1g16670 are shown.
Line ID 878D10
Locus At1g16670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5698605
Primers used for PCR 12sv - 8474, show primer details
   
Sequence Name 37-K116654-0022-878-D10C-12sv
GenBank Accession LN502752 [GenBank]
Sequencing primer gene specific primer, 12sv: 5'-ACTATCTCCATCAGAAGGACTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116654-0022-878-D10C-12sv
GCATACTCTGGTGCTAATAACCACTGCCGAGAAACAGATGTGAACACATATAAAGACCTG
CAATTTTCAGAGGGAAATAAGAAAAAGAGGGAAAAGAAATACATGGAACTCACATTGTAC
CCGCGACACGAGTGCTAACATGAGTCATGTTAGGTGGCATAAGTCTAGCAAGTCCAAAAT
CAGAGATCTTGGGGGATAGATATTTGTCAAGTAGAATGTTGCTTGCTTTGATATCTCTGT
GAATTATGTGTGGGCGTACTTCCTCATGAAGAAAGGCAGGACAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGAA
TATTAT
   
Sequence Name 12-K116653-0022-878-D10C-8409
GenBank Accession LN491751 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116653-0022-878-D10C-8409
ACATTGAATAATCCTGTCCTGCCTTTCTTCATGAGGAAGTACGCCCACACATAATTCACA
GAGATATCAAAGCAAGCAACATTCTACTTGACAAATATCTATCCCCCAAGATCTCTGATT
TTGGACTTGCTAGACTTATGCCACCTAACATGACTCATGTTAGCACTCGTGTCGCGGGTA
CAATGTGAGTTCCATGTATTTCTTTTCCCTCTTTTTCTTATTTCCCTCTGAAAATTGCAG
GTCTTTATATGTGTTCACATCTGTTTCTCGGCAGTGGTTATTTAGCACCAGAGTATGCGG
TTAGGGGACAGTTGACGCGTAAAGCTGATATATACAGCTTTGGAGTCCTTCTAGGGGAGA
TAGT

Last Updated on 06.12.2018 16:11