Sequences from confirmation amplicon


Confirmation sequences for line 878E11 and hit At3g24500 are shown.
Line ID 878E11
Locus At3g24500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8918823
Primers used for PCR KR13 - 8474, show primer details
   
Sequence Name 31-K116569-0022-878-E11C-KR13
GenBank Accession LN500312 [GenBank]
Sequencing primer gene specific primer, KR13: 5'-AAACTCTAATTGTGTTCACCCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K116569-0022-878-E11C-KR13
GTTTTATGACATTCCATATCATTTTAACAGTTACAATCACAAAACCGTACCGTACTTTTT
ACCGCGCTTAAAGCTTTTTTTTGGTTGCTAACAATAAAGGTATACGNGTTTTATGTCATC
AATATAACTATATATAAAAGAAATGAAAGATATATATTGTTTTTTCATTTATCAAACAAA
ACAACAAGACTTTTTTTTTACTTTTTACATTGGTCAACAAAATACAAGATAAACGACATC
GTTTAATCATTTCCCAATTTTACCCCTAAGTTTAACACCTAGAACCTTCTCCATCTTCGC
AAGCACAGCCTGATTAGGAACAGCTTTACCATTCTCATATTCCTGAACTACCTGAGTCCT
CTCATTGATCTGTTTCGCCAAATCCGCTTGTGACATCTTCTTCTCCAATCTCGCTTTCTG
TATCATCAACCTCACCTCTGCTTTCACACGATCCATCGCCGCAGGCTCTGTTTCTTCTTC
CAGCTTCTTCGTGTTAATCACCGGAACCGCCGTAGATTTCCCCTTTTTGTTCGAACCGGC
ATCGAATTTCTTAACCGTTTGAACCGCGACACCGTTTCTCAGAGCTGCGTTAACCGCTTT
CGGATCGCGTAGGTCTTGGCTCTTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATG
   
Sequence Name 02-K116568-0022-878-E11C-8409
GenBank Accession LN487195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116568-0022-878-E11C-8409
TTCAAAGAGCCAAGACCTACGCGATCCGAAAGCGGTTAACGCAGCTCTGAGAAACGGTGT
CGCGGTTCAAACGGTTAAGAAATTCGATGCCGGTTCGAACAAAAAGGGGAAATCTACGGC
GGTTCCGGTGATTAACACGAAGAAGCTGGAAGAAGAAACAGAGCCTGCGGCGATGGATCG
TGTGAAAGCAGAGGTGAGGTTGATGATACAGAAAGCGAGATTGGAGAAGAAGATGTCACA
AGCGGATTTGGCGAAACAGATCAATGAGAGGACTCAGGTAGTTCAGGAATATGAGAATGG
TAAAGCTGTTCCTAATCAGGCTGTGCTTGCGAAGATGGAGAAGGTTCTAGGTGTTAAACT
TAGGGGTAAAATTGGGAAATGATTAAACGATGTCGTTTATCTTGTATTTTGTTGACCAAT
GTAAAAAGTAAAAAAAAAGTCTTGTTGTTTTGTTTGATAAATGAAAAAACAATATATATC
TTTCATTTCTTTTATATATAGTTATATTGATGACATAAAACCGTATACCTTTATTGTTAG
CAACCAAAAAAAAGCTTTAAGCGCGGTAAAAAGTACGGTACGGTTTTGTGATTGGTAACT
GTTAAAATGATATGGAATTGTCAATCAAAACCATCATTTTTAGATTTTAACATTTTTGTG
GGGTGAACCA

Last Updated on 06.12.2018 16:11