Sequences from confirmation amplicon


Confirmation sequences for line 878F05 and hit At1g23200 are shown.
Line ID 878F05
Locus At1g23200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8229325
Primers used for PCR 08py - 8474, show primer details
   
Sequence Name 38-K115371-0022-878-F05C-08py
GenBank Accession LN503096 [GenBank]
Sequencing primer gene specific primer, 08py: 5'-GACTAATTTTTGTTTTATCCGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115371-0022-878-F05C-08py
CATTCGAGAACCGGCAGGACCGGAGAAACATCAGGCGGTGGCTCTCCGATCAAGTTCAGA
CTTCTCCGTCTTCTACGCCTGCTCTTTCAAAGGCTATCAAGACACTCTCTACCTTCATTC
TAGTCGTCAGTTCTTGAGAAACTGCAATATTTACGGAACCGTCGATTTCATCTTCGGAGA
CGCAACCGCCATCCTCCAAAACTGTAACATCTACGCTCGTAAGCCGATGAGCGGCCAGAA
AAACACGATCACCGCTCAATCACGCAAAGAACCCGACGAGACTACTGGCTTCGTCATCCA
GAGCTCGACGGTGGCTACTGCGTCGGAAACTTATTTAGGACGGCCGTGGAGATCGCATTC
GAGGACGGTTTTCATGAAATGTAATCTTGGAGCGTTGGTGAGTCCGGCGGGATGGTTGCC
TTGGAGTGGTAGTTTTGCTCTTAGCACTCTTTATTACGGTGAGTATGGTAATACCGGCGC
CGGAGCAAGTGTCTCCGGTAGAGTTAAGTGGCCAGGTTATCATGTTATAAAGACGGTGAC
AGAGGCGGAAAAATTCACGGTGGAGAATTTCTTGGACGGAAACTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 18-K115370-0022-878-F05C-8409
GenBank Accession LN494433 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115370-0022-878-F05C-8409
TTTTTCCGCCTCTGTCACCGTCTTTATAACATGATAACCTGGCCACTTAACTCTACCGGA
GACACTTGCTCCGGCGCCGGTATTACCATACTCACCGTAATAAAGAGTGCTAAGAGCAAA
ACTACCACTCCAAGGCAACCATCCCGCCGGACTCACCAACGCTCCAAGATTACATTTCAT
GAAAACCGTCCTCGAATGCGATCTCCACGGCCGTCCTAAATAAGTTTCCGACGCAGTAGC
CACCGTCGAGCTCTGGATGACGAAGCCAGTAGTCTCGTCGGGTTCTTTGCGTGATTGAGC
GGTGATCGTGTTTTTCTGGCCGCTCATCGGCTTACGAGCGTAGATGTTACAGTTTTGGAG
GATGGCGGTTGCGTCTCCGAAGATGAAATCGACGGTTCCGTAAATATTGCAGTTTCTCAA
GAACTGACGACTAGAATGAAGGTAGAGAGTGTCTTGATAGCCTTTGAAAGAGCAGGCGTA
GAAGACGGAGAAGTCTGAACTTGATCGGAGAGCCACCGCCTGATGTTTCTCCGGTCCTGC
CGTGTTCTCGAATGTTATGCCTTGTGCGATAAAACCGTTGCCGGAAACAGCTGTTGGCGG
ATAAA

Last Updated on 06.12.2018 16:11