Sequences from confirmation amplicon


Confirmation sequences for line 880F04 and hit At5g01560 are shown.
Line ID 880F04
Locus At5g01560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:218771
Primers used for PCR 73wl - 8474, show primer details
   
Sequence Name 75-K118414-0022-880-F04C-73wl
GenBank Accession LN513303 [GenBank]
Sequencing primer gene specific primer, 73wl: 5'-AATTCTGGTGAGAGACGAAGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K118414-0022-880-F04C-73wl
GACGAGTTTCGATCCTAAATCTGTTCTTGAAGAATCTGAATATCCCCAATTGTCGTGAAT
CTCAGGAACATCTTCGTCTCTGTTCAGGTACATGAGCACCATTCTCATCAGCGGCCGAGA
TTCCGGTTTGTGGTGACAACAGAGCAAACCGACGGCGAGAGCTAGCCTTGCCTCTCCTTC
GTCGTAACCAGATCCGAGTCTCGGGTCGATCGCACTGAGGATCTCACCGCTCGCTTGTAG
CTCCATAACCCAGTCGGCTATAAAGAAGGTCCCAGAGTCCGTCGGTTTCCTCCCGGAGAC
GATCTCTAACAGCAAAACGCCAAACGCGAAAACGTCGGAAGCTGATGAGGAGTTTCCGTT
GCGGGCTAGCTCCGGCGCCATATATCCAATCGTGCCGACGACTACGGTGGTGCACGATTG
AGATCCGCGTTCGTAAAGCCTCGCGAGTCCAAAGTCTCCCAATCTAGGATTCATATCGGA
GTCAATCAGAACATTGCTAGGTTTCATGAAGCCATTTTTGGGGCATGATTGGGGCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGC
   
Sequence Name 27-K118413-0022-880-F04C-oriL
GenBank Accession LN498600 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118413-0022-880-F04C-oriL
ATTTACAATTGAATTATCCTGCCCCAATCATGCCCCAAAAATGGCTTCATGAAACCTAGC
AATGTTCTGATTGACTCCGATATGAATCCTAGATTGGGAGACTTTGGACTCGCGAGGCTT
TACGAACGCGGATCTCAATCGTGCACCACCGTAGTCGTCGGCACGATTGGATATATGGCG
CCGGAGCTAGCCCGCAACGGAAACTCCTCATCAGCTTCCGACGTTTTCGCGTTTGGCGTT
TTGCTGTTAGAGATCGTCTCCGGGAGGAAACCGACGGACTCTGGGACCTTCTTTATAGCC
GACTGGGTTATGGAGCTACAAGCGAGCGGTGAGATCCTCAGTGCGATCGACCCGAGACTC
GGATCTGGTTACGACGAAGGAGAGGCAAGGCTAGCTCTCGCCGTCGGTTTGCTCTGTTGT
CACCACAAACCGGAATCTCGGCCGCTGATGAGAATGGTGCTCAGGTACCTGAACAGAGAC
GAAGATGTTCCTGAGATTCACGACAATTGGGGATATTCAGATTCTTCAAGAACAGATTTA
GGATCGAAACTCGTCGGATACATTTCGTCGGATAGGGCTTCGAGCTCACACTCACACACA
TCTTCGTCTC
Primers used for PCR DR40 - 8474, show primer details
   
Sequence Name 74-K118414-0022-880-F04C-DR40
GenBank Accession LN513148 [GenBank]
Sequencing primer gene specific primer, DR40: 5'-ATAAACCAAGAATCCCCTTGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118414-0022-880-F04C-DR40
TCGTCGGATTCACAGCAGCGACTGGGGAGACACGGACAATCGAGTGCTCATTACGTCATG
GGTTGGAGTTTCGCAAGCGGTGGAGAACATCCACTTGCAGCTATGCTCGACATCTCCCAA
CTTCCTCCTCCGCCTCCGAATAAGGCGAAGAAGAGAGGTTACAATGGTAAGGTCATCGCT
CTTATCGTGGCTTTATCGACTGTTATATCGATCATGTTGGTGTTGCTGTTCCTCTTCATG
ATGTACAAAAAACGAATGCAGCAAGAAGAGATTCTAGAAGATTGGGAAATCGATCATCCT
CACAGATTTCGATACAGAGACCTCTACAAGGCTACTGAGGGATTCAAGGAGAATAGGGTT
GTCGGAACCGGAGGATTCGGAATTGTTTACAGAGGAAACATCAGATCATCGTCTGATCAA
ATCGCAGTGAAGAAGATAACCCCAAATAGCATGCAAGGTGTTCGAGAATTTGTCGCGGAG
ATTGAGAGTTTGGGGAGATTAAGGCATAAGAATCTGGTGAACCTCCAAGGATGGTGTAAA
CACAGAAACGATCTCTTGTTGATCTACGATTATATTCCCAACGGAAGCTTGGACTCGCTG
CTTTACAGTAAACCGAGAAGAAGCGGAGCCGTTTTGTCGTGGAACGCGCGTTTTCAAATC
GCTAAAGGAATCGCGTCTGGTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTA
   
Sequence Name 26-K118413-0022-880-F04C-8409
GenBank Accession LN498132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118413-0022-880-F04C-8409
ATTTACAAACAGACGCGATTCCTTTAGCGATTTGAAAACGCGCGTTCCACGACAAAACGG
CTCCGCTTCTTCTCGGTTTACTGTAAAGCAGCGAGTCCAAGCTTCCGTTGGGAATATAAT
CGTAGATCAACAAGAGATCGTTTCTGTGTTTACACCATCCTTGGAGGTTCACCAGATTCT
TATGCCTTAATCTCCCCAAACTCTCAATCTCCGCGACAAATTCTCGAACACCTTGCATGC
TATTTGGGGTTATCTTCTTCACTGCGATTTGATCAGACGATGATCTGATGTTTCCTCTGT
AAACAATTCCGAATCCTCCGGTTCCGACAACCCTATTCTCCTTGAATCCCTCAGTAGCCT
TGTAGAGGTCTCTGTATCGAAATCTGTGAGGATGATCGATTTCCCAATCTTCTAGAATCT
CTTCTTGCTGCATTCGTTTTTTGTACATCATGAAGAGGAACAGCAACACCAACATGATCG
ATATAACAGTCGATAAAGCCACGATAAGAGCGATGACCTTACCATTGTAACCTCTCTTCT
TCGCCTTATTCGGAGGCGGAGGAGGAAGTTGGGAGATGTCGAGCATAGCTGCAAGTGGAT
GTTCTCCACCGCTTGCGAAACTCCAACCCATGACGTAATGAGCACTCGATTGTCCGTGTC
TCCCAGTCGCTGCTGTGAATCCGACGAACATTTCGTCTACGACAATATCTGACAGTTTTG
GAACCTCTCGTGAGATCAAGG

Last Updated on 06.12.2018 16:11