Genotyping and primer details


Genotyping and primer details for 880F04 - At5g01560
Line ID 880F04
Locus At5g01560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:218771 of line 880F04 Chr5:218771
   
Insertion allele amplicon 8474 - 73wl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 73wl: 5'-AATTCTGGTGAGAGACGAAGATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:218200
   
Insertion allele amplicon 8474 - DR40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DR40: 5'-ATAAACCAAGAATCCCCTTGATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:219569
   
wt amplicon (over InsPos) CN30 - EU91
wt amplicon start Chr5:218268
wt amplicon end Chr5:219208
wt amplicon size 941 bp
Locus specific primer EU91: 5'-TCATCCTCACAGATTTCGATACAG-3'
2nd locus specific primer CN30: 5'-CGAGTTTCGATCCTAAATCTGTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11