Sequences from confirmation amplicon


Confirmation sequences for line 884A06 and hit At3g11930 are shown.
Line ID 884A06
Locus At3g11930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3777257
Primers used for PCR NF67 - 8474, show primer details
   
Sequence Name 35-K102502-0022-884-A06C-8409
GenBank Accession LN501782 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102502-0022-884-A06C-8409
GCCATTTACAATTGAAATATATAAGAAGAAGTTATTAATGATACAATACCTTTTGATCTT
GCCAAGGCCACGACTACCCACAACGAGAAGATCAACGTGCATTTTCTCCACCGCCTCACA
AATCATCTCCTTCGCCTCGCCTTCTAGCACTAGAGTTTCAGTACGTATCTGCACATCCAT
CACACAATATGTTTTAATCGCGTTTCATGTCCAATAATTAGTGTAATGTCTTTAGGACAT
GTGTTATAGAATCGGACCTGCTTGGCTCGGCACATTTGGAGAGCGCGAGAGAGAAGGGCA
GCAGAGGTCTCTTGTTGTGCCTTTTTCACTGACTCTATCATTGATGAAGATGCGTACACT
GCTGCTACAACCGTTCACAAAACCAGNTCATAC
   
Sequence Name 35-K102503-0022-884-A06C-NF67
GenBank Accession LN501783 [GenBank]
Sequencing primer gene specific primer, NF67: 5'-GTGTATGACTTGGTTTTGTGAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102503-0022-884-A06C-NF67
TAGAGTCAGTGAAAAAGGCACAACAAGAGACCTCTGCTGCCCTTCTCTCTCGCGCTCTCC
AAATGTGCCGAGCCAAGCAGGTCCGATTCTATAACACATGTCCTAAAGACATTACACTAA
TTATTGGACATGAAACGCGATTAAAACATATTGTGTGATGGATGTGCAGATACGTACTGA
AACTCTAGTGCTAGAAGGCGAGGCGAAGGAGATGATTTGTGAGGCGGTGGAGAAAATGCA
CGTTGATCTTCTCGTTGTGGGTAGTCGTGGCCTTGGCAAGATCAAAAGGTATTGTATCAT
TAATAACTTCTTCTTATATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11