Sequences from confirmation amplicon


Confirmation sequences for line 885D07 and hit At1g08520 are shown.
Line ID 885D07
Locus At1g08520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2696415
Primers used for PCR 02lu - 8474, show primer details
   
Sequence Name 46-K114669-0022-885-D07C-02lu
GenBank Accession LN505945 [GenBank]
Sequencing primer gene specific primer, 02lu: 5'-AGCAGTTTTTATGCCTTCCTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114669-0022-885-D07C-02lu
CGGCATCTAACAAGTTGAACAAACAAATGTAAAAGTCCCATAACACACAGTTCACTGATG
AAAAACTGAAGCTTTTTCCTATACCCAACTCCAACTAAATCAAATTGAGTAGAATTATGC
AACCTGCAGAATCAAATTGCAAATTCTCTGACCTGGCCAACAACTGCGGCCAAAGGGAAA
AACTGTCGGCCATAGGAGGTGGTATCCGTCTCCGTATCCGTATCTGATGTGGAGGCAGGG
ACACCGTTAGGGGATTCGACGGTAGCATTCGCGGAGGCACGTACAGTGAAGCGGCACGAG
GCAATTCTGTTTCGTTTGGGGAGGGAGAGAAACAGAGGCTTAGGTAAGAGATCAGGGAGG
AGATTGCAGCGAAAGAGTCTGCAGGTTGAAACTGGAGATGATGACGCGACCGGAGTCATC
GCCATTTTCAAAGATNGGAGAAGAAGAAGAAGAAGAAGAAGAAGTTAAGAGATAGTATTT
TCTACTGTATCANACCTTATCAGCTTACTTTTGCTTCTCTATCTNTTGTCTCGTCATCCT
TTCCTGATTGCAGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT
   
Sequence Name 23-K114668-0022-885-D07C-8409
GenBank Accession LN496660 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114668-0022-885-D07C-8409
TGCATCAGGAAAGATGACGAGACAAAGATAGAGAAGCAAAAGTAAGCTGATAAGGTTTGA
TACAGTAGAAAATACTATCTCTTAACTTCTTCTTCTTCTTCTTCTTCTTCTCCTATCTTT
GAAAATGGCGATGACTCCGGTCGCGTCATCATCTCCAGTTTCAACCTGCAGACTCTTTCG
CTGCAATCTCCTCCCTGATCTCTTACCTAAGCCTCTGTTTCTCTCCCTCCCCAAACGAAA
CAGAATTGCCTCGTGCCGCTTCACTGTACGTGCCTCCGCGAATGCTACCGTCGAATCCCC
TAACGGTGTCCCTGCCTCCACATCAGATACGGATACGGAGACGGATACCACCTCCTATGG
CCGACAGTTTTTCCCTTTGGCCGCAGTTGTTGGCCAGGTCAGAGAATTTGCAATTTGATT
CTGCAGGTTGCATAATTCTACTCAATTTGATTTAGTTGGAGTTGGGTATAGGAAAAAGCT
TCAGTTTTTCATCAGTGAACTGTGTGTTATGGGACTTTTACATTTGTTTGTTCAACTTGT
TAGATGCCGAAAGCCTCACAGAGGATGTAATTTGTTTGCTTTAACTAAATGTTGTTTGTA
GGA

Last Updated on 06.12.2018 16:11