Sequences from confirmation amplicon


Confirmation sequences for line 888E01 and hit At1g66150 are shown.
Line ID 888E01
Locus At1g66150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24634120
Primers used for PCR 14pf - 8474, show primer details
   
Sequence Name 48-K115781-0022-888-E01C-14pf
GenBank Accession LN506601 [GenBank]
Sequencing primer gene specific primer, 14pf: 5'-GGTCTAGTGGGAATACTCATCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K115781-0022-888-E01C-14pf
AGATCACTTCTTCCTTCTAAGCTTGCCATTTCTTAAGTGCTTGAGGTAAAGACATGTCGA
GATCGATACCATATATGTCTTCTGGATTCTGATCCGACGGTTTCCATAGCTCAACGAGTG
ATGACAGAATGTTGACTGCGTGTCCCATGTCTGGTCTCTGGTAAGGCTCACGGGCACAGC
AATGGCTTACCAACAGAAAAAGAAACCTTCCTGTAATATGGATCAGCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 24-K115780-0022-888-E01C-8409
GenBank Accession LN497131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115780-0022-888-E01C-8409
AGGAAGGTTTCTTTTTCTGTTGGTAAGCCATTGCTGTGCCCGTGAGCCTTACCAGAGACC
AGACATGGGACACGCAGTCAACATTCTGTCATCACTCGTTGAGCTATGGAAACCGTCGGA
TCAGAATCCAGAAGACATATATGGTATCGATCTCGACATGTCTTTACCTCAAGCACTTAA
GAAATGGCAAGCTTATGAAGGAAGAAGTGATCTCGAATCTTCAACTTCATCACTTTTACC
TAGCTTGGACAACACGCAGATGAGTATTCCCACTAGAC

Last Updated on 06.12.2018 16:11