SimpleSearch - Line and FST details


Line specific information

 
Line ID 888E01
Vector Used pAC161
Line Availability available as T3 set from NASC (N485201)
Segregation Analysis N/A (line seemingly not resistant, T2 plants grown without drug)
Confirmed for Hit At1g66150
Parent of DUPLO pair none
Parent of pair(s) 3469, 3527, 7154

Gene hit At1g66150

 
Sequence (A. th genome BLAST matches underlined) 
>05-K113049-0022-888-E01-8409
AGCCATTTACATTGAATATATCCTGCAATGGTCTTCGCAACAGTGTGAACGCTGGCTAAG
GTTTCTTCGTCTAACCTGCCCGGCCACTAANCGTAATTTGTCGTTTTATCAAAATGTACT
TTCATTTTATAATAACGCTGCGGACATCTACTTTTTTGAATTGAAAAAAAATTGGTAATT
ACTCTTTCTTTTTCTCCATATTGACCATCATACTCATTGCTGATCCATGTAAATTTCCCG
GACATGAAGCCATTTACAATTGAATATATCCTGCAATGGTCTTCGCAACAGTGTGAACGC
TGGCTAAGGTTTCTTCGTCTAACATACCCGGCCG
GenBank Accession FR817194 [GenBank]
Graphic View Graphic view of gene At1g66150
Predicted Position of Insertion Chr1:24634353 - go to primer design
BLAST e Value 7e-12
Hit Clone Code (BAC ID) F15E12
Hit Gene Code At1g66150 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation transmembrane kinase 1
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit FR817193 [GenBank] FR817192 [GenBank] FR817194 [GenBank]

Gene hit At2g17470

 
Sequence (A. th genome BLAST matches underlined) 
>05-K030272-022-888-E01-8409
AGCCATATACACCTGAACGTATGCGTATCCTCCCTATAGTGAGCTCCCCCCCCGCGTCGC
AAAAACAACCACCTCCCCCCGAGGTATGCCTTCCTTCAATCGAGACGCAAAACATATTGC
GCTTTTCGCAAGCAAACTTTTGCAAATTCACACTCGATTCTTAACTTAAAAGATAATCAT
AAACTTTTATAAAGTATCTCCAACATGTTGCATTATTATATTGNATGGGATCCTCCCTAT
ANTGAGACNTACTAC
GenBank Accession CR935323 [GenBank]
Graphic View Graphic view of gene At2g17470
Predicted Position of Insertion Chr2:7584396 - go to primer design
BLAST e Value 3e-34
Hit Clone Code (BAC ID) F5J6
Hit Gene Code At2g17470 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation aluminum activated malate transporter family protein
Insertion Classification TS2TE (3')
Confirmation Status unknown
Other FSTs Supporting this Hit CR935323 [GenBank]


Last Updated on 10.06.2021 13:37