Sequences from confirmation amplicon


Confirmation sequences for line 888G12 and hit At2g31070 are shown.
Line ID 888G12
Locus At2g31070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13220919
Primers used for PCR 10ib - 8474, show primer details
   
Sequence Name 24-K112877-0022-888-G12C-8409
GenBank Accession LN497066 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112877-0022-888-G12C-8409
GAAACCGACAATAATTAAATCTTTCATAATTAAATTACCACATGTATTTCTTTCAGACAA
CCATGGAGGTTCAAATTCATGATGACATGCATTGAACAAGAAGGAGGAAATTAATGTGAT
GAATGGAGCTAGAGTAAGCGCTATATTAATTAGTATTACCATCTATCCATATATTTATGA
TTCAAGACTATTATTACTCATTTAGTGTTTGTTATTATTATTCAATTTTGCTTGTTTGTT
TCTTATCATCTTTGCATTGATCATCAAGATTGTGATATTTGAAAAGATGGGTTTTATGTG
TGTTTTGGTTTAGTACTGGCGTTAGGCTGGGATATTGTAATCTCATATTTTTGTTCTACG
AGTGAGTTTGAATTTTCTGGTTTTTACTTGCGGTTTCTATTTCTTCGGTTAAGTAAAGAG
TAATTGAAGAACGTTAAAAGCATAGGTCG
   
Sequence Name 61-K112878-0022-888-G12C-10ib
GenBank Accession LN510565 [GenBank]
Sequencing primer gene specific primer, 10ib: 5'-CGACCTATGCTTTTAACGTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K112878-0022-888-G12C-10ib
AACCGCAAGTAAAACCAGAAAATTCAAACTCACTCGTAGAACAAAAATATGAGATTACAA
TATCCCAGCCTAACGCCAGTACTAAACCAAAACACACATAAAACCCATCTTTTCAAATAT
CACAATCTTGATGATCAATGCAAAGATGATAAGAAACAAACAAGCAAAATTGAATAATAA
TAACAAACACTAAATGAGTAATAATAGTCTTGAATCATAAATATATGGATAGATGGTAAT
ACTAATTAATATAGCGCTTACTCTAGCTCCATTCATCACATTAATTTCCTCCTTCTTGTT
CAATGCATGTCATCATGAATTTGAACCTCCATGGTTGTCTGAAAGAAATACATGTGGTAA
TTTAATTATGAAAGATTTAATTATTGTCTGGTTTCTTGATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11