Sequences from confirmation amplicon


Confirmation sequences for line 888G12 and hit At3g02750 are shown.
Line ID 888G12
Locus At3g02750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:593870
Primers used for PCR 12hz - 8474, show primer details
   
Sequence Name 13-K112993-0022-888-G12C-12hz
GenBank Accession LN492116 [GenBank]
Sequencing primer gene specific primer, 12hz: 5'-TCTGTAACTGGAATTGGAGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112993-0022-888-G12C-12hz
AATGTCCACAGACGACAAATTAGCCTGAGGGGATTCACAGAATTACAGACACAAGCCATT
ATTACATGATGTGATGTTTTTTCACTCTTCTTTACTGACAAGACCAAAAAACAACATAAA
CATTAACAAGAGAGAAAGACATAAACCAAAGACAAAACTGACAAAAATTTAGGTTCCAAA
ACCAATGAATTATGACTCAAAAAAGAATAGAGATCAATGAAGAAAGTTGAAAAGGAAAAA
CAAAAGAACAAAAAGACACAGTACCTTGTCTTGGGTGATTTATTTGATATCATTAATCAC
CTCTCAACTTCTTCACTTCAACTCTTCTTTCTCTATTCACTTTCCAGGCACAAATCTTGG
TAAGTTTAAAAGTGTATTAACTCTTGCAACACCTTCAAGTGCAGAATACTCTGTTCCAGG
TTCTGAATCCAAGTTATCCGCTTCTTTTATCAGCTTCTCAGTTTCACTTTCGTCGAGAGC
AATCTCTTTCCCCGACCTGACAGTACTCGAACGGCCTAGACCGCTGGGTCCTGATGCATC
ATCATCCTCAGTCGTGGCTTTTAGTTCTTCTTCTTCTCCATCTTCAAGTTTGGAGATGGA
AGAAGCTGTAGATATGGCGTTTGTGTTGCTGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACG
GTTATT
   
Sequence Name 03-K112992-0022-888-G12C-8409
GenBank Accession LN487563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112992-0022-888-G12C-8409
GCTTCTTCATCTCCAACTTGAAGATGGAGAAGAAGAAGAACTAAAAGCCACGACCGATGA
TGATGATGCATCAGGACCAAGCGGTCTAGGCCGTTCGAGTACTGTCAGGTCGGGGAAAGA
GATTGCTCTCGACGAAAGTGAAACTGAGAAGCTGATAAAAGAAGCGGATAACTTGGATTC
AGAACCTGGAACAGAGTATTCTGCACTTGAAGGTGTTGCAAGAGTTAATACACTTTTAAA
CTTACCAAGATTTGTGCCTGGAAAGTGAATAGAGAAAGAAGAGTTGAAGTGAAGAAGTTG
AGAGGTGATTAATGATATCAAATAAATCACCCAAGACAAGGTACTGTGTCTTTTTGTTCT
TTTGTTTTTCCTTTTCAACTTTCTTCATTGATCTCTATTCTTTTTTGAGTCATAATTCAT
TGGTTTTGGAACCTAAATTTTTGTCAGTTTTGTCTTTGGTTTATGTCTTTCTCTCTTGTT
AATGTTTATGTTGTTTTTTGGTCTTGTCAGTAAAGAAGAGTGAAAAAACATCACATCATG
TAATAATGGCTTGTGTCTGTAATTCTGTGAATCCCCTCAGGCTTAATTTTGTCAGTCTGT
TGGGACAATTTCCTGTTTCTGTCTTTTCAAAAAGCTCCAATTCCGGTTACAG

Last Updated on 06.12.2018 16:11