Sequences from confirmation amplicon


Confirmation sequences for line 888H01 and hit At1g10010 are shown.
Line ID 888H01
Locus At1g10010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3266906
Primers used for PCR 16hz - 8474, show primer details
   
Sequence Name 43-K112562-0022-888-H01C-16hz
GenBank Accession LN504890 [GenBank]
Sequencing primer gene specific primer, 16hz: 5'-CCTATTCCGATAGACGCATAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112562-0022-888-H01C-16hz
GCTTGTGGAAGTAGGAAGCTGGCTCAGAATGATCTGGACGATCCCAAATGCCGCCATGTA
TGGATAATTCGATACAGAACATTTCGCTTTATGTCCCTTGTCATGATAACAATTTGATTT
CCCAATCGCTCTAACGAAGACGTACAAAAACACCAATCAAACAATTATTAGTAATTTTAT
TCAGAACAACATGTTAGTATAACCATAACAAAATAATCGAAATAAATCAGAATCTTTGAC
TTACACTAAGCTTATGGAGGCAGTGATGGTGTAACCAATAGTGACCCCTACGAGATTCAC
GTACTGTGCCACTCCACATAGCTGAACCTTTTTACCACCTGCGTTTTATGTTATTAAAGA
AGAAAAATGATTACTCTTGTACTACTATCAAATACTACCAATCTTTTGGTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K112561-0022-888-H01C-8409
GenBank Accession LN494827 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112561-0022-888-H01C-8409
GCATTTACNATTGACCAAAAGATTGGTAGTATTTGATAGTAGTACAAGAGTAATCATTTT
TCTTCTTTAATAACATAAAACGCAGGTGGTAAAAAGGTTCAGCTATGTGGAGTGGCACAG
TACGTGAATCTCGTAGGGGTCACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTG
TAAGTCAAAGATTCTGATTTATTTCGATTATTTTGTTATGGTTATACTAACATGTTGTTC
TGAATAAAATTACTAATAATTGTTTGATTGGTGTTTTTGTACGTCTTCGTTAGAGCGATT
GGGAAATCAAATTGTTATCATGACAAGGGACATAAAGCGAAATGTTCTGTATCGAATTAT
CCATACATGGCGGCATTTGGGATCGTCCAGATCATTCTGAGCCAGCTTCCTAACTTCCAC
AAGCTCTCTTTCCTATCCATCATCGCCGCGGTTATGTCCTTCTCTTATGCGTCTACGGGA
ATAGG

Last Updated on 06.12.2018 16:11