Sequences from confirmation amplicon


Confirmation sequences for line 889B02 and hit At2g35270 are shown.
Line ID 889B02
Locus At2g35270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14857708
Primers used for PCR 16my - 8474, show primer details
   
Sequence Name 34-K114505-0022-889-B02C-16my
GenBank Accession LN501509 [GenBank]
Sequencing primer gene specific primer, 16my: 5'-TTAACCCAATAGAAGAAACCATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K114505-0022-889-B02C-16my
GTTTTTTTTCTTAATCAAATTTCATAAAATTCGGAGAAAACAAAAAAGTGGAAAAAACAA
TTAAAGTTTCTTAAATTGCTAAAACGGAGCCCTACCGGCGCCGGCGTGATTCCCCTGCCA
ACTTTCAACTCCAATCTGAGGCATACTCATCGGCAAATTAAAGAAAGGCAACCCTCCGCC
ACCGCCAGTGGCTTCCGAGTACATATTCCCTCCACCTCCGCCGCCGCCACTTTGCAAGTG
CTCCTCATGCTCGTCCAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGC
   
Sequence Name 09-K114504-0022-889-B02C-8409
GenBank Accession LN490286 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114504-0022-889-B02C-8409
CATGAGAGCACTTGCAAAGTGGCGGCGGCGGAGGTGGAGGGAATATGTACTCGGAAGCCA
CTGGCGGTGGCGGAGGGTTGCCTTTCTTTAATTTGCCGATGAGTATGCCTCAGATTGGAG
TTGAAAGTTGGCAGGGGAATCACGCCGGCGCCGGTAGGGCTCCGTTTTAGCAATTTAAGA
AACTTTAATTGTTTTTTCCACTTTTTTGTTTTTCTCCGAATTTTATGAAATTATGATTTA
AGAAAAAAAACGATATTGTTCATGTATTGACCCTCTTACTGCATGGTTTCTTCTATTGGG
TTA

Last Updated on 06.12.2018 16:11