Sequences from confirmation amplicon


Confirmation sequences for line 891C10 and hit At1g04170 are shown.
Line ID 891C10
Locus At1g04170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1099687
Primers used for PCR 24uf - 8474, show primer details
   
Sequence Name 40-K117042-0022-891-C10C-24uf
GenBank Accession LN503843 [GenBank]
Sequencing primer gene specific primer, 24uf: 5'-TATACACAGTGGAAGGCAAGACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117042-0022-891-C10C-24uf
TGAACATTTTGACTAAAAAGTCGTATTTATTATAAAAAGTCGTACTTGATCGGATCCTAA
TGAGTAATGAAGTTGTTCCTTTTCTGAAGAAAGTATGTATAGTCAAGTAGCAGAGTATCT
CTGACAAAAATTTCAAAAAAAAAAGGAGGGCCACCAAATTTTGTTATTTTCTAAAAAAAA
ACCCCAACCAAGGGGCAACCTGTTACCATTACCAAAGAGCAACCTGTTACCATTACCAAT
CCGGGTCCGGTTGAAAAATTTTAAACCCCAAAAAACAAACTCCCCAAAACCCCCCACCAA
AAAAAAAATTTTTATTATCTTTTGGAGGGGTTAAACATTTTTTTTTCCAAAACCCCAACC
AAAAGGGACAAAATTACCCCTTGCTTAAAAAGGGGGAGGGAGGACCTCCTGGAAATGGGT
TCCTGGCCGGGAAATCCTCCTGGGATCACCATGGATTTGGAGGGCCAATTGGGAAAAAAA
AAAAAAGTATTTCCCATTTTTTTTCCATTTCAAAATGGTAAATTCCCC
   
Sequence Name 10-K117041-0022-891-C10C-8409
GenBank Accession LN490833 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117041-0022-891-C10C-8409
TCCTTCCCTTTCTAGCTATGTGTTATTTTTGTCCTTTTTGTTTGTGTTTCTGAAAAAAAG
ATTGTTAAACCCCTCAAAAAGATAATAAAACTCTTTTTCTCGTTGGGGGATTTTGGAGAG
TTCGTCTTTGCGGATTAAACATCTTCAAACAGTACCTGATTTGTTATTGTTACTAGTTTG
CTCTTTGTTATTGTTACTAGTTTGCTCTTTGTTTGTGTTTTTCTCTTAGATATTACTAAA
TTTTGTTGTCTCTCTTTTTTTTTTGAAAATTTTTGCCAAAAAAACCCGGCAACTTGACAA
AACAAACTTTCTTCAAAAAGGGAACACTTTCTTTCCTCTTTAGGACCCAATCAATTCCAA
TTTTTTAAAAAAAATACAATTTTTTATCCAAAAGGTTCAGGTCTCCCCTCAAAAAAACTT
TTTTTGTTTGCAATTTAAATTGAGTGTCTGGCCTCCCACTGGGAAAAA

Last Updated on 06.12.2018 16:11