Sequences from confirmation amplicon


Confirmation sequences for line 891H09 and hit At5g13580 are shown.
Line ID 891H09
Locus At5g13580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4372604
Primers used for PCR 45wz - 8474, show primer details
   
Sequence Name 34-K118804-0022-891-H09C-45wz
GenBank Accession LN501607 [GenBank]
Sequencing primer gene specific primer, 45wz: 5'-TCCAATTCAACAATTTATCAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118804-0022-891-H09C-45wz
ACAAGAGATAAAGAAAATACGAAAACAAACAAAGGTAAATCAAGTTTCAAACCAGTCCTC
CATAAGATCAAACTGAGTTTTGTTTTTACCTCCTCTTGTTCTTGCTACCCAAAAGCAGAG
AAAAGTAGAACAAGATTCGAAAGAAGAAGCCCCAAGCGACCGTGACCCACAAGCAATTCC
ATTTGGTGAGATCCGTAACGCCTTGTTGCTGCAAAATGTCATATCCTGTGGTCAAGCAAG
TAGAGCTTGTGATCCTCATTCCAAGTGATTTGCTCATAGTTGCTAGAAGCCTAACTTTCA
TCCCCTGAGGCACCGCGACCAGAGGTGTGTTGTCGAATATCTGAACTCCTCTGACGAAAC
ACTTTGTTGGGTCACCGAATTCGTTTAGGAGCACAGCCTCATAAGGATACTTGACTAAGG
AAATGTAATGGAACCAGATCCAGTAGCCAGGGATTCGATCTCGGTTGATGAAGAATCCAC
TGAAAAGCAAGAAATAGGCTAAAATGTAGCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 15-K118803-0022-891-H09C-8409
GenBank Accession LN493196 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118803-0022-891-H09C-8409
CATTTCATTGAATATATCCTGGCTACATTTTAGCCTATTTCTTGCTTTTCAGTGGATTCT
TCATCAACCGAGATCGAATCCCTGGCTACTGGATCTGGTTCCATTACATTTCCTTAGTCA
AGTATCCTTATGAGGCTGTGCTCCTAAACGAATTCGGTGACCCAACAAAGTGTTTCGTCA
GAGGAGTTCAGATATTCGACAACACACCTCTGGTCGCGGTGCCTCAGGGGATGAAAGTTA
GGCTTCTAGCAACTATGAGCAAATCACTTGGAATGAGGATCACAAGCTCTACTTGCTTGA
CCACAGGATATGACATTTTGCAGCAACAAGGCGTTACGGATCTCACCAAATGGAATTGCT
TGTGGGTCACGGTCGCTTGGGGCTTCTTCTTTCGAATCTTGTTCTACTTTTCTCTGCTTT
TGGGTAGCAAGAACAAGAGGAGGTAAAAACAAAACTCAGTTTGATCTTATGGAGGACTGG
TTTGAAACTTGATTTACCTTTGTTTGTTTTCTGTATTTTTCTTTTATCTCTTTGTTGTAT
ATTCTTATATATGTGGTTGATAAATTGTGGAATTGGA
Primers used for PCR 50xh - 8474, show primer details
   
Sequence Name 25-K119080-0022-891-H09C-50xh
GenBank Accession LN497683 [GenBank]
Sequencing primer gene specific primer, 50xh: 5'-CAACAAAGGGTTTAGACAGAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119080-0022-891-H09C-50xh
CTTAAAGAGCCATCAGCGCAAGTATCTCTAAAGGCAAGCTCGTCTCTGGAGCGACCACAA
CAACTCATAGCTCTGGCTCGAGCCCTGTTTCCACTATTCCAACTTTCGCGAACCCGTTTT
GGGTCGAACTCGCGGTTCTAGCCAAACGGTCCATGACTAATTCTCGCAGACAGCCTGAAC
TATTCGGGATACGCCTAGGAGCTGTTTTAGTCACTGGATTCATCTTAGCCACTATGTTTT
GGCAGCTAGATAACTCCCCTAAAGGAGTCCAAGAACGTCTTGGATGCTTCGCTTTTGCAA
TGTCCACAACGTTCTACACTTGCGCAGATGCTCTCCCTGTTTTCCTCCAAGAACGTTTCA
TTTTCATGAGAGAAACTGCTTACAACGCTTACCGAAGATCCTCCTATGTACTATCTCACT
CTCTTGTTGCCCTCCCTTCCTTGATCATCCTCTCGCTTGCTTTTGCAGCGATCACATTTT
GGGGTGTGGGTTTAGATGGAGGTCTTATGGGTTTCCTCTTCTACTTCCTAGTAATCTTAG
CCTCTTTCTGGGCAGGAAGCTCATTCGACACCTTCTTATCAGGCGTTGGGCATGATATAT
TCAATTGTAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCGATGAGTATGATGGT
CGATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTAT
   
Sequence Name 11-K119079-0022-891-H09C-8409
GenBank Accession LN491347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K119079-0022-891-H09C-8409
CATTGAATATATCCTGCCCAACGCCTGATAAGAAGGTGACGAATGAGCTTCCTGCCCAGA
AAGAGGCTAAGATTACTAGGAAGTAGAAGAGGAAACCCATAAGACCTCCATCTAAACCCA
CACCCCAAAATGTGATCGCTGCAAAAGCAAGCGAGAGGATGATCAAGGAAGGGAGGGCAA
CAAGAGAGTGAGATAGTACATAGGAGGATCTTCGGTAAGCGTTGTAAGCAGTTTCTCTCA
TGAAAATGAAACGTTCTTGGAGGAAAACAGGGAGAGCATCTGCGCAAGTGTAGAACGTTG
TGGACATTGCAAAAGCGAAGCATCCAAGACGTTCTTGGACTCCTTTAGGGGAGTTATCTA
GCTGCCAAAACATAGTGGCTAAGATGAATCCAGTGACTAAAACAGCTCCTAGGCGTATCC
CGAATAGTTCAGGCTGTCTGCGAGAATTAGTCATGGACCGTTTGGCTAGAACCGCGAGTT
CGACCCAAAACGGGTTCGCGAAAGTTGGAATAGTGGAAACAGGGCTCGAGCCAGAGCTAT
GAGTTGTTGTGGTCGCTCCAGAGACGAGCTTGCCTTTAGAGATACTTGCGCTGATGGCTT
CTTTTAAGGACAAGCCAGTCTGACTTCTTGGCTCTGCTTTCCTCTGTCTAACCCCTTTGT
TG

Last Updated on 06.12.2018 16:11