Sequences from confirmation amplicon


Confirmation sequences for line 893B07 and hit At5g40510 are shown.
Line ID 893B07
Locus At5g40510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16230108
Primers used for PCR CN70 - 8474, show primer details
   
Sequence Name 55-K104163-0022-893-B07C-CN70
GenBank Accession LN508796 [GenBank]
Sequencing primer gene specific primer, CN70: 5'-AGATCGCAAATCTGATCTTCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K104163-0022-893-B07C-CN70
AAGATTCTCATTAGTCATTACGTTGACTTTTTTTGTTGTTGTTGTTGTAGACGAAGCTCA
ATGTATGTGAAGGTGGTGGATCTGACGGTGATGTTTTGATTTTTCCCGATATGATCAGAT
ACAAGTGAGTGTGCTTAAGTTTTGTTTTTGTTAGCTTAAGATTTTTGGAAAAAAAATAGT
TGACTAGAATAGGATTTTGACTCTTAGCTAGATTTATAGTGTGTAAATTGTTGTATAAGC
TAGTTTTAGAAACTTTAGAAGTTAGATTATAGATCTTAGTATATTTCTCAGATCTATGAG
ATCCAAATTTGCATTTTGTGATTTTGTTTAAACTATTATTCGTGTTGGTATTGACAATAG
GGGGGTTAAGGATACGGATGTGGAAGGTTTCTTTGAAGATGTTCTTGTGAATGGGAAGCC
ATGGAGTTCTGGGATACAAGAAGAGATATCTGGAACATTTGTATTTGTGTGTACTCATGC
TAGTCGTGACAAGAGATGTGGTGTTTGTGGACCAGTGATCTTGGAGAGGTTTAAGCAAGA
GATTGGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
   
Sequence Name 55-K104164-0022-893-B07C-8409
GenBank Accession LN508797 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K104164-0022-893-B07C-8409
ATTTACATTGAAACCAATCTCTTGCTAAACCTCTCCAAGATCACTGGTCCACAAACACCA
CATCTCTTGTCACGACTAGCATGAGTACACACAAATACAAATGTTCCAGATATCTCTTCT
TGTATCCCAGAACTCCATGGCTTCCCATTCACAAGAACATCTTCAAAGAAACCTTCCACA
TCCGTATCCTTAACCCCCCTATTGTCAATACCAACACGAATAATAGTTTAAACAAAATCA
CAAAATGCAAATTTGGATCTCATAGATCTGAGAAATATACTAAGATCTATAATCTAACTT
CTAAAGTTTCTAAAACTAGCTTATACAACAATTTACACACTATAAATCTAGCTAAGAGTC
AAAATCCTATTCTAGTCAACTATTTTTTTTCCAAAAATCTTAAGCTAACAAAAACAAAAC
TTAAGCACACTCACTTGTATCTGATCATATCGGGAAAAATCAAAACATCACCGTCAGATC
CACCACCTTCACATACATTGAGCTTCGTCTACAACAACAACAACAAAAAAAGTCAACGTA
ATGACTAANGAGAATCTTCATCAGAAAGAGATACAACGTAAGAAAATGTCAAACGTTTTA
GTATAAACCTGAACAAGAAGAT

Last Updated on 06.12.2018 16:11