Sequences from confirmation amplicon


Confirmation sequences for line 894C07 and hit At1g27820 are shown.
Line ID 894C07
Locus At1g27820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9691669
Primers used for PCR 23sr - 8474, show primer details
   
Sequence Name 74-K116549-0022-894-C07C-23sr
GenBank Accession LN513138 [GenBank]
Sequencing primer gene specific primer, 23sr: 5'-TGGCTTTTAAGAGAAAAATGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K116549-0022-894-C07C-23sr
AGGAAATTTTGTGTTGTGCCTTAAAAAAAAAAGGAAACTGAAGTCTGTGTTACTCTACAT
AATCTAATCTCCTCTAACAACAATCCTAATAACCAAACGAAAATAAATTCAGGTGGCTTC
TCGCTTTTCTCTTTCAGAAAAACAAACGTTTTTTTCTTTCGTAGTTTCGTTCGAGATGAA
GCAAATTAGTAGCGGTGAAGTTTGGAGATGGAACAAGAAAGAAAGACATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 34-K116548-0022-894-C07C-8409
GenBank Accession LN501562 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K116548-0022-894-C07C-8409
TACATGTCTTTCTTTCTTGTTCATCTCCAACTTCACCGCTACTAATTTGCTTCATCTCGA
ACGAAACTACGAAAGAAAAAAACGTTTGTTTTTCTGAAAGAGAAAAGCGAGAAGCCACCT
GAATTTATTTTCGTTTGGTTATTAGGATTGTTGTTAGAGGAGATTAGATTATGTAGAGTA
ACACAGACTTCAGTTTCCTTTTTTTTTTAAGGCACAACACAAAATTTCCTTTTTGTTTCT
CCTAATTCTTGTTAAACACGAGAATTTCCATTTTTCTTTTAAAAGCCAA

Last Updated on 06.12.2018 16:11