Sequences from confirmation amplicon


Confirmation sequences for line 895B09 and hit At3g13080 are shown.
Line ID 895B09
Locus At3g13080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4199536
Primers used for PCR KM81 - 8474, show primer details
   
Sequence Name 79-K038032-022-895-B09C-KM81
GenBank Accession LN513783 [GenBank]
Sequencing primer gene specific primer, KM81: 5'-ATCTCCAGATCCTTACTCAACGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K038032-022-895-B09C-KM81
ATCGTTCTCTTTCATAGGTTTGCCAACAAGATGTTATCCTCAATTTTACCACTCTGAATC
CAAGGAGATTGCGCAACATACGCTTTTGTCCCACAAACCTTAAGACTTCCAGATACCTTG
GGTACTTCTCCTAGTAAAGATGAAAGTAAGCTCGATTTCCCAGAGCCAACGGTACCACAA
ACTGCAACCTTCATCCCAGGAAAGACCTTGAAATTTATGTCTTTGAGAGTCGGGTTAGAG
GATGAGACATCCCAAGATAAAGTGCTGTTGATCACTTCTACTGCTACGTCTGAACTTCCC
TTAGGAAGCCTCTCTACAATGTCAGGCTGCAAATTGTCCAGGCTATGGTAGGATGCAAGT
CTATCAATATAGACTATAGTCTGCACAAACATGGAGATATTGT
   
Sequence Name 23-K038032-022-895-B09C-8409
GenBank Accession LN496471 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K038032-022-895-B09C-8409
GCCATTTCAATTGAATCCCCCTTGAATCAGGAAAGATACTCTCTGCACTTGCAACCTTCA
GGATTCTACAAGAGCCGATCTACAATCTTCCCGACACTATCTCCATGATTGTGCAGACTA
AAGTCTCTCTTGATAGACTTGCATCCTACCTTTGCCTGGACAATTTGCAGCCTGACATTG
TAGAGAGGCTTCCTAAGGGAAGTTCAGACGTGGCAGTAGAAGTGATCAACAGCACTTTAT
CTTGGGATGTCTCATCCTCTAACCCGACTCTCAAAGACATAAATTTCAAGGTCTTTCCTG
GGATGAAGGTTGCAGTTTGTGGTACCGTTGGCTCTGGGAAATCGAGCTTACTTTCATCTT
TACTAGGAGAAGTACCCAAGGTATCTGGAAGTCTTAAGGTTTGTGGGACAAAAGCGTATG
TTGCGCAATCTCCTTGGATTCAGAGTGGTAAAATTGAGGATAACATCTTGTTTGGCAAAC
CTATGGAAAGAGAACGATATGATAAGGTGCTTGAAGCATGTTCGTTGAGTAA

Last Updated on 06.12.2018 16:11