Sequences from confirmation amplicon


Confirmation sequences for line 895G06 and hit At1g48240 are shown.
Line ID 895G06
Locus At1g48240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17811254
Primers used for PCR 25fx - 8474, show primer details
   
Sequence Name 75-K111632-0022-895-G06C-25fx
GenBank Accession LN513264 [GenBank]
Sequencing primer gene specific primer, 25fx: 5'-TTCAATTTTTCATTCGAGTGTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K111632-0022-895-G06C-25fx
ATTTTTTGGGACTGATTTGTAGGAGATTGAATTTGAGTTTCACAGACGAGAAAATAAGAG
TTGGGATAGTGAATCTGAGGCACAAGTCTTATGATTTTTTTCTTTAAAAGTGAGATTCAA
CATTGATATGCAGGAATGGCTTCCAGAGGTTGGATAAGATCAAGGATTCTAGTAGACAAA
GCAAGCAACTCGAAGAACTTGCTGAGAAGATGAGAGACTGTAAAAGGTATTCACTTTCTC
CAAGATATCAGTTTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCG
   
Sequence Name 59-K111631-0022-895-G06C-8409
GenBank Accession LN510036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K111631-0022-895-G06C-8409
ATTGAAAAAAACTGATATCTTGGAGAAAGTGAATACCTTTTACAGTCTCTCATCTTCTCA
GCAAGTTCTTCGAGTTGCTTGCTTTGTCTACTAGAATCCTTGATCTTATCCAACCTCTGG
AAGCCATTCCTGCATATCAATGTTGAATCTCACTTTTAAAGAAAAAAATCATAAGACTTG
TGCCTCAGATTCACTATCCCAACTCTTATTTTCTCGTCTGTGAAACTCAAATTCAATCTC
CTAACAAATCTAGTTCCCAAAAAATCTATAGTCTTGTCGATAACGTACACTCGAATGAAA
AATTGA

Last Updated on 06.12.2018 16:11