Sequences from confirmation amplicon


Confirmation sequences for line 895G12 and hit At4g13070 are shown.
Line ID 895G12
Locus At4g13070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7622353
Primers used for PCR CB07 - 8474, show primer details
   
Sequence Name 66-K104528-0022-895-G12C-CB07
GenBank Accession LN511604 [GenBank]
Sequencing primer gene specific primer, CB07: 5'-TGTGTCTTTCTTTCTGTGTGCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K104528-0022-895-G12C-CB07
ATTCTTCTTGCTTCACTATCAATCCTCTAATTACTCCAGTTATGTAAAGTCAAGACCTTT
TCTACAAGTTCAATGTAAATCAGCTTCTTTTGATGTTTCACAAGCTCAGAGATGGAATCT
ATTTGCGTATGATGGTTTGTTTAGTAGACGTTACTTGAGTAATTCCACAATAGAGCTAAG
AACAGATGATAATGTGGTTAGGTTTGCTTTCAACAATGTCTCTAGTAGTGGTAACAATGT
TGTACCTACAAGAAAAGAGAAGAAATGGAAACGGGCTAAATTGTCAAGAAAAGCTAAAGT
GAATGAGTTAAGGTTTTATCGTCTGAAGGCTAAAAAGAAGATGAATTCTCCAAACCCTGA
AGTTAGAATTCGTTACAAGCTTGAAAAGGTTACGTCTTAACATTGTATTAGTCAATGTAA
CATGTTAGTAGCTTTGGAGTTTATTTAAAATCTGTTGGTGTGGTTGATTGTGTAGGCTAA
GAGGAAAGAAGAATGGTTGATTGAGAAACTGAGGAAATACGATGTTCCGAAATCACCAAT
TTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 82-K104529-0022-895-G12C-8409
GenBank Accession LN514190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K104529-0022-895-G12C-8409
ATTGGTGATTTCGGAACATCGTATTTCCTCAGTTTCTCAATCAACCATTCTTCTTTCCTC
TTAGCCTACACAATCAACCACACCAACAGATTTTAAATAAACTCCAAAGCTACTAACATG
TTACATTGACTAATACAATGTTAAGACGTAACCTTTTCAAGCTTGTAACGAATTCTAACT
TCAGGGTTTGGAGAATTCATCTTCTTTTTAGCCTTCAGACGATAAAACCTTAACTCATTC
ACTTTAGCTTTTCTTGACAATTTAGCCCGTTTCCATTTCTTCTCTTTTCTTGTAGGTACA
ACATTGTTACCACTACTAGAGACATTGTTGAAAGCAAACCTAACCACATTATCATCTGTT
CTTAGCTCTATTGTGGAATTACTCAAGTAACGTCTACTAAACAAACCATCATACGCAAAT
AGATTCCATCTCTGAGCTTGTGAAACATCAAAAGAAGCTGATTTACATTGAACTTGTAGA
AAAGGTCTTGACTTTACATAACTGGAGTAATTAGAGGATTGATAGTGAAGCAAGAAGAAT
CTAGAGGTCCTGCAAAAAACACAGAAACATATTTATAAGAGCACACAG
Primers used for PCR 17wq - 8474, show primer details
   
Sequence Name 33-K119006-0022-895-G12C-17wq
GenBank Accession LN501201 [GenBank]
Sequencing primer gene specific primer, 17wq: 5'-CCATAAGACCATACATTGGTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K119006-0022-895-G12C-17wq
ACGACTAAACTAGTAAAAGTTGTATATAGACTTCTCTATTCTAATTCTCTAACCGGNTTG
GTATAACTTGGTTTAGCCAAACTGAACCCATTTACTTAGACATAATGCAATGTTCACCGT
TGCTTTCAGAATCCTTTTGAGAATTGGAATCTTGATGCTACAAAAACTCTGAAACGAGAG
ACCTTACACTACTTTGCTCTTCGAAACCGCCTCTTTCTTCTTCTCTGCTTCTTCATCTTT
CTTCTTCTTGTGCCGAGCAACGTACTTATGGTACTCCTCAAGCTCCTTCTCCAGCTTCTC
TATGAACTCACTCGTATGTTCAAGCGATTGCTCATACCGATATTTCTCCAAAGCCTGACG
TTTTCATTCACTTTTGTCAAGACAAGAAACAACCAGCCAAAAATCAAGTATCTAGATTCA
ACAATATACCTTATCTTTAGAGAGAGTATCAACAGGAGACATCACTTCTGGTCTCACATA
GTTTTTCCCACGGTACAACACAATGGTGTTGTTAGGTTTGACATCAATCACAATGCCCTT
ACTTAACCGAGCAAGCTCTTCTGCATACTCATGAACCTGCCCGGGTTTGTTGCAGGGCTT
GCAAATAACTTTAACAGTCTCATGCTTTTTCCAATGAAGGTGCAAATTCAAAACCACACC
TCCAAACACTCCTCGTCTTCCGACAAGTACAAAGTTCTTTCTCTTCTCACCTGTACGTTT
TAAGTAATGCTGCTCTTCCTCTGTCAAACTCTCTGGAGAAATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGGTCATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTCAATTCAAA
   
Sequence Name 03-K119005-0022-895-G12C-8409
GenBank Accession LN487700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119005-0022-895-G12C-8409
ATTTCTCCAGAGAGTTTGACAGAGGAAGAGCAGCATTACTTAAAACGTACAGGTGAGAAG
AGAAAGAACTTTGTACTTGTCGGAAGACGAGGAGTGTTTGGAGGTGTGGTTTTGAATTTG
CACCTTCATTGGAAAAAGCATGAGACTGTTAAAGTTATTTGCAAGCCCTGCAACAAACCC
GGGCAGGTTCATGAGTATGCAGAAGAGCTTGCTCGGTTAAGTAAGGGCATTGTGATTGAT
GTCAAACCTAACAACACCATTGTGTTGTACCGTGGGAAAAACTATGTGAGACCAGAAGTG
ATGTCTCCTGTTGATACTCTCTCTAAAGATAAGGTATATTGTTGAATCTAGATACTTGAT
TTTTGGCTGGTTGTTTCTTGTCTTGACAAAAGTGAATGAAAACGTCAGGCTTTGGAGAAA
TATCGGTATGAGCAATCGCTTGAACATACGAGTGAGTTCATAGAGAAGCTGGAGAAGGAG
CTTGAGGAGTACCATAAGTACGTTGCTCGGCACAAGAAGAAGAAAGATGAAGAAGCAGAG
AAGAAGAAAGAGGCGGTTTCGAAGAGCAAAGTAGTGTAAGGTCTCTCGTTTCAGAGTTTT
TGTAGCATCAAGATTCCAATTCTCAAAAGGATTCTGAAAGCAACGGTGAACATTGCATTA
TGTCTAAGTAAATGGGTTCAGTTTGGCTAAACCAAGTTATACCAACCGGTTAGAGAATTA
GAATAGAGAAGTCTATATACAACTTTTACTAGTTTAGTCCTTTGTCAATAAATATCACCA
TTCAGTTATTTTGTATGGAGAACCACCAATGTATGGTCTTATG

Last Updated on 06.12.2018 16:11