Sequences from confirmation amplicon


Confirmation sequences for line 897B12 and hit At1g66960 are shown.
Line ID 897B12
Locus At1g66960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24988386
Primers used for PCR 48vy - 8474, show primer details
   
Sequence Name 24-K118107-0022-897-B12C-48vy
GenBank Accession LN497177 [GenBank]
Sequencing primer gene specific primer, 48vy: 5'-TGAACGTTGATGGTAATCCTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118107-0022-897-B12C-48vy
TTATGCTCAAAGATTCACCGAGAGCCAAACTTTTCCCCAACAAGGAATATAAATCACACC
GCCATGGCTAAGAATCCATTGCCTGGCCCGTTTGCATGCGTTTTCTAGTCCTCCATCGGG
ACCTACTCCAAGTATACGCAAGCATATGTAATTCAGCGTGGTAGTGAACATAATGCTTTT
GCTCTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACGGATTATA
   
Sequence Name 05-K118106-0022-897-B12C-8409
GenBank Accession LN488544 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118106-0022-897-B12C-8409
CATTTCATTGATATTGAGAGCAAAGCATTATGTTCACTACCACGCTGAATTACATATGCT
TGCGTATACTTGGAGTAGGTCCCGATGGAGGACTAGAAAACGCATGCAAACGGGCCAGGC
AATGGATTCTTAGCCATGGCGGTGTGATTTATATTCCTTGTTGGGGAAAAGTTTGGCTCT
CGGTGAATCTTTTGGAGCATAAGTTAATTTATAGCTTAGAGATGTTGAAACTTGTAGGAT
TACCATCAACGTTCAAAGGATGCGG

Last Updated on 06.12.2018 16:11