Sequences from confirmation amplicon


Confirmation sequences for line 897G01 and hit At5g27100 are shown.
Line ID 897G01
Locus At5g27100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9536574
Primers used for PCR LB36 - 8474, show primer details
   
Sequence Name 23-K033540-022-897-G01C-8409
GenBank Accession LN496458 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K033540-022-897-G01C-8409
ATTTACATTGAATAATCCTGCTCATAACCACCATATTGACCATTTATTTTTCTTTATCTG
CTCAATCTTTACTAAGATGGGTTGTTGAAACCACGTCTGTGTTCTTAATGTTTGGAGCAG
AAATATGATGCTGTTGTGGCAGATACAACAATATCGTCAAATAGGTCAATGTATGTTGAT
TTCTCATTGCCATACACACCATCAGGCGTAGGTCTTGTCGTCCCTGTTAAAGACAGTGTT
AGAAGAAGCAGTACCATCTTTTTGATGCCTTTGACTTTGGCACTTTGGCTAATCAGCCTT
TTGTCCTTTTTCATTATTGGTCTTGTCGTATGGGTTCTTGAACATAGGGTAA
   
Sequence Name 23-K033540-022-897-G01C-LB36
GenBank Accession LN496459 [GenBank]
Sequencing primer gene specific primer, LB36: 5'-TTTACCCTATGTTCAAGAACCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K033540-022-897-G01C-LB36
AAGGCTGATTAGCCAAGTGCCAAAGTCAAAGGCATCAAAAAGATGGTACTGCTTCTTCTA
ACACTGTCTTTAACAGGGACGACAAGACCTACGCCTGATGGTGTGTATGGCAATGAGAAA
TCAACATACATTGACCTATTTGACGATATTGTTGTATCTGCCACAACAGCATCATATTTC
TGCTCCAAACATTAAGAACACAGACGTGGTTTCAACAACCCATCTTAGTAAAGATTGAGC
AGATAAAGAAAAATAAATGGTCAATATGGTGGTTATGAGCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
T

Last Updated on 06.12.2018 16:11