Sequences from confirmation amplicon


Confirmation sequences for line 897H04 and hit At5g37060 are shown.
Line ID 897H04
Locus At5g37060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14643775
Primers used for PCR KQ79 - 8474, show primer details
   
Sequence Name 13-K101499-0022-897-H04C-8409
GenBank Accession LN491972 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101499-0022-897-H04C-8409
AGTAGTCCCTTATCTATATTCGCAGTCCAGCTAGTGGAACTAGCAGGAAGAGCCACNGCC
GTTGTTTATTGACCACGAACAACGAAAGGAGGAGGAAGAAGAAGAATACGAGGAGGAAGA
GGAAGAGCCTGAACGTAAGCAAAGTGGACGGATAGATCAGGTGCAAAGCGCCTTTAAGTT
GTACGAAGAGAAAAGAAATGAGTGCGTGACGTTGCGTTCATACACAGCTCATGCTCCAAA
ACGTCTCATGTACCAAGACATTTGCGAGCTTGCCTTAGGCAAGAAAACCGCTTTCATTCT
CTTACCTTATCAGAAAGAGCGTCTTGAAGACGCTGCCCCTACCGAGCTACGTGATTCCGG
CATGCTATCTGTGAACGCAGACGTCTTGGAACACACACCTTGCTCTGTCTGTATATATNT
TGACAAGGGACGGCTTAAGAACGCGGTGGTTAGGTTATCCATGGACCTACNACACTCAAC
AAACTCAATCCGTATGAGGCAAGAAACGTACCGTTTCGTGGTTTTGTTTTTAGGAGGAGC
TGATAACAGAGAAGCTCT
   
Sequence Name 13-K101503-0022-897-H04C-KQ79
GenBank Accession LN491973 [GenBank]
Sequencing primer gene specific primer, KQ79: 5'-CGGCTAGATGTAGAGCTTCTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101503-0022-897-H04C-KQ79
GTACGTTTCTTGCCTCATACGGATTGAGTTTGTTGAGTGTTGTAGGTCCATGGATAACCT
AACCACCGCGTTCTTAAGCCGTCCCTTGTCAAAATATATACAGACAGAGCAAGGTGTGTG
TTCCAAGACGTCTGCGTTCACAGATAGCATGCCGGAATCACGTAGCTCGGTAGGGGCAGC
GTCTTCAAGACGCTCTTTCTGATAAGGTAAGAGAATGAAAGCGGTTTTCTTGCCTAAGGC
AAGCTCGCAAATGTCTTGGTACATGAGACGTTTTGGAGCATGAGCTGTGTATGAACGCAA
CGTCACGCACTCATTTCTTTTCTCTTCGTACAACTTAAAGGCGCTTTGCACCTGATCTAT
CCGTCCACTTTGCTTACGTTCAGGCTCTTCCTCTTCCTCCTCGTATTCTTCTTCTTCCTC
CTCCTTTCGTTGTTCGTGGTCAATAAACAACGGCGTGGCTCTTCCTGCTAGTTCCACTAG
CTGGACTGCGAATATNGATAAGGGACTACTACATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11