Sequences from confirmation amplicon


Confirmation sequences for line 899A04 and hit At1g75020 are shown.
Line ID 899A04
Locus At1g75020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28172509
Primers used for PCR 36ve - 8474, show primer details
   
Sequence Name 19-K117874-0022-899-A04C-36ve
GenBank Accession LN494946 [GenBank]
Sequencing primer gene specific primer, 36ve: 5'-GTTCTTCTTCTGGTCTTTGATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117874-0022-899-A04C-36ve
TTTGTCCTTCACTTAATGAATCCATTAACCAAGCAGAAGATTCTGCCTCGTTTGCTGGAA
TCTCCTTTAGTAGAACTCGCCGAACGTGGATATGTACTTCTGAAGGATCCGTCCCGAATA
CATTGTCCATGAAAGATGGGCAGCGAGGTTTATATGCGATAGTCAAATCATATACTGCAT
TGCAATAAAAGGAAATGCCTTGTTCAGTTAATTGTGGTCATGAGGTCCCACATGGGTCAT
TGGAAAAATAGAAGATATGCACGCCATCATAATGTCGTCAAAACACATAAGTGTTTGCAT
AACATGTGGCTTGAGATACTACTATATGTACACCAACATTTGTAACAAAATTTCATGAAC
CATACCTGCATCCAAAGAGTTATGAAGAACTTCTAAGCAAACGCCAAAACCCCTTGTTTT
TGGTAGAAGTACGTTTGATAGTGCTGGAAGACCAACTTCAGCTGCAAACTTTTGGCTTCT
CTTGCATTTCTCTTCACTGCATATCAAATAAGGAAGGTCTCAGTAATAGAAAGAAGATAT
ACTAAGAGCAGCATTTCAAACATTCATGCAATAGCAGGCAAATTATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 07-K117873-0022-899-A04C-8409
GenBank Accession LN489459 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117873-0022-899-A04C-8409
CCATTTCATAATTTGCCTGCTATTGCATGAATGTTTGAAATGCTGCTCTTAGTATATCTT
CTTTCTATTACTGAGACCTTCCTTATTTGATATGCAGTGAAGAGAAATGCAAGAGAAGCC
AAAAGTTTGCAGCTGAAGTTGGTCTTCCAGCACTATCAAACGTACTTCTACCAAAAACAA
GGGGTTTTGGCGTTTGCTTAGAAGTTCTTCATAACTCTTTGGATGCAGGTATGGTTCATG
AAATTTTGTTACAAATGTTGGTGTACATATAGTAGTATCTCAAGCCACATGTTATGCAAA
CACTTATGTGTTTTGACGACATTATGATGGCGTGCATATCTTCTATTTTTCCAATGACCC
ATGTGGGACCTCATGACCACAATTAACTGAACAAGGCATTTCCTTTTATTGCAATGCAGT
ATATGATTTGACTATCGCATATAAACCTCGCTGCCCATCTTTCATGGACAATGTATTCGG
GACGGATCCTTCAGAAGTACATATCCACGTTCGGCGAGTTCTACTAAAGGAGATTCCAGC
AAACGAGGCAGAATCTTCTGCTTGGTTAATGGATTCATTTAAGTTGAAGGACAAACTGCT
ATCCGATTTCAATGCTCAGGGGAAATTCCCCAATCAAAG

Last Updated on 06.12.2018 16:11