Sequences from confirmation amplicon


Confirmation sequences for line 900B12 and hit At3g01320 are shown.
Line ID 900B12
Locus At3g01320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:109333
Primers used for PCR 35kk - 8474, show primer details
   
Sequence Name 33-K113872-0022-900-B12C-35kk
GenBank Accession LN501077 [GenBank]
Sequencing primer gene specific primer, 35kk: 5'-GAGAAAGAAAGTGAACACAAGCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K113872-0022-900-B12C-35kk
GACATGCAGAAGGAGTACATGGGAAAATCTATTCAGAGCTCGATCTCTCTGATTGCGAGT
GTTGCACTCCTAGCTACCGGCTTCTGCCTGCGGATGTAAGCTTCTATTTCCAAGTTGCAT
TTTGAGCATGTTCATTGGATTTTTACTTACTGTAATATTATATTTGCAGTATCCGATACC
AATTGCGAGTCAGAGATCGGAGCTAGGAGCTGAGGTTTTAAATGATCATTGGGTATCTGT
CACTTCCGGAAGTGAAGATTATTCTTTTAAGCACATGCGCAGAAACCAATATGAAGAGAG
CTTGTTCAGATGCGAAGATGATAGTAATTGTAAATTCAATATGGATCAGCAATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 07-K113871-0022-900-B12C-8409
GenBank Accession LN489357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K113871-0022-900-B12C-8409
TCATCTTCGCATCTGAACAAGCTCTCTTCATATTGGTTTCTGCGCATGTGCTTAAAAGAA
TAATCTTCACTTCCGGAAGTGACAGATACCCAATGATCATTTAAAACCTCAGCTCCTAGC
TCCGATCTCTGACTCGCAATTGGTATCGGATACTGCAAATATAATATTACAGTAAGTAAA
AATCCAATGAACATGCTCAAAATGCAACTTGGAAATAGAAGCTTACATCCGCAGGCAGAA
GCCGGTAGCTAGGAGTGCAACACTCGCAATCAGAGAGATCGAGCTCTTGAATAGATTTTC
CCATGTACTCCTTCTTGCATTGCTCAGTTTCCTTCACAGCCTCGAGTTCAGGCTTGTGTT
CACTTTCTTTCT

Last Updated on 06.12.2018 16:11