Sequences from confirmation amplicon


Confirmation sequences for line 900D02 and hit At1g69828 are shown.
Line ID 900D02
Locus At1g69828 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26288048
Primers used for PCR 38kk - 8474, show primer details
   
Sequence Name 74-K113845-0022-900-D02C-38kk
GenBank Accession LN513116 [GenBank]
Sequencing primer gene specific primer, 38kk: 5'-GGGTTCTAACAAAGTCTCCTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113845-0022-900-D02C-38kk
TTTGTGAAAAAAACTTCAATAAGAAAATGGCATCTAACAAAGTCTCATTTTTCTGGGTTC
TTTGTCTATGTATTCTCTTAGCCGGAGGTGAGCGATTTCTTTGACATCTAGCTATTAATT
TCCCTATAAATAACCTTACAATTTGATATATATAGCTAAATTTATTTATTTTATCTGAAA
CTCCTTATAAAATATACTTGGGGAGTTTGTCTGATTTACCGAAAATATCATTTGTTACTT
TTTCATATAACTAATAATTCTGTTAATCTTTTGTAACGTAAATTGACAGAGTGTATAGAA
TCACCAATTTTCACCGGAAACAAATGCTCAAATCCAACCGGCATGGACAAAGATGGAAAA
TGTCTTGATTACTGTCATGCTCAAGGTTATCCCGGGGGTTCTTGTATAGGCTTCATCGAT
CAAGGTTATATGTGTGTATGTAAAGTAGGATAAAACTGATTAAAATTGCTACTCACATTT
AAACATGGATGGATCTCTTCTCTGGGATACATGTTCTTTTCAATTTCGTATCATAAAATA
TTAAGAATAACTAAGAGAAAAAGTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 47-K113844-0022-900-D02C-8409
GenBank Accession LN506258 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113844-0022-900-D02C-8409
CATTTACATTGAATATATCCTGCAACTTTTTCTCTTAGTTATTCTTAATATTTTATGATA
CGAAATTGAAAAGAACATGTATCCCAGAGAAGAGATCCATCCATGTTTAAATGTGAGTAG
CAATTTTAATCAGTTTTATCCTACTTTACATACACACATATAACCTTGATCGATGAAGCC
TATACAAGAACCACCGGGATAACCTTGAGCATGACAGTAATCAAGACATTTTCCATCTTT
GTCCATGCCGGTTGGATCTGAGCATTTGTTTCCGGTGAAAATTGGTGATTCTATACACTC
TGTCAATTTACGTTACAAAAGATTAACAGAATTATTAGTTATATGAAAAAGTAACAAATG
ATATTTTCGGTAAATCAGACAAACTCACCAAGTATATTTTATAAGGAGTTTCAGATAAAA
TAAATAAATTTAGCTATATATATCAAATTGTAAGGTTATTTATAGGGAAATTAATAGCTA
GATGTCAAAGAAATCGCTCACCTCCGGCTAAGAGAATACATAGACAAAGAACCAAGAAAA
ATGAGACTTTGTTAGATGCCATTTTCTTATTTGAAGTTTTTTTTTTTCACAAACTACAAA
ATATATGAATTTAGATAAGGAAGGAGAC

Last Updated on 06.12.2018 16:11