Sequences from confirmation amplicon


Confirmation sequences for line 900E09 and hit At5g10260 are shown.
Line ID 900E09
Locus At5g10260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3221269
Primers used for PCR 13xh - 8474, show primer details
   
Sequence Name 22-K119080-0022-900-E09C-13xh
GenBank Accession LN496335 [GenBank]
Sequencing primer gene specific primer, 13xh: 5'-GGACCTCCTAACTACACACAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K119080-0022-900-E09C-13xh
GCAACAGTAAAGAGCTCTAATGATTTGTTGATCTGATATCTTACTCTTACATTTAATGTG
ATACTAGTAATAAACTAATAATAGATTAATATGAGAAACTGAATCTCTTTCTTTTCCCAA
TTCTTTCCAAGCTCTTATTTCGTTATCCAAACATTTTCTAGTAATGTCAAGATGGAGACT
GACAGTGTGTGATGTAAAAGAAATGAGCAATGAGAACAGAGCAAAACATGCCATTCTTCT
TCATAAGCTTTCAGACGTCTTTATTACACAACTCTATATTGAAAACAAAACCTCTCCAAA
GAAAAACTAGAGTCTCCAAGTGTAATCACCTTTCCTCATACCAGGATTTATTTGTTCCAT
AAAATTATGAATCTTTCGTTTAAAAATCTTCCTATTCTGCTGCTGTGTATATATTTAAAA
CTGATTAACAAGCACAACCACCTCGTTGTTGCTCCCCAATTCCCATTGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTAT
   
Sequence Name 08-K119079-0022-900-E09C-8409
GenBank Accession LN489953 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119079-0022-900-E09C-8409
CATTGAATATATCAATGGGAATTGGGGAGCAACAACGAGGTGGTTGTGCTTGTTAATCAG
TTTTAAATATATACACAGCAGCAGAATAGGAAGATTTTTAAACGAAAGATTCATAATTTT
ATGGAACAAATAAATCCTGGTATGAGGAAAGGTGATTACACTTGGAGACTCTAGTTTTTC
TTTGGAGAGGTTTTGTTTTCAATATAGAGTTGTGTAATAAAGACGTCTGAAAGCTTATGA
AGAAGAATGGCATGTTTTGCTCTGTTCTCATTGCTCATTTCTTTTACATCACACACTGTC
AGTCTCCATCTTGACATTACTAGAAAATGTTTGGATAACGAAATAAGACTTGGAAAGAAT
TGGGAAAAGAAAGAGATTCAGTTTCTCATATTAATCTATTATTAGTTTATTACTAGTATC
ACATTAAATGTAAGAGTAAGATATCAGATCAACAAATCATTAGAGCTCTTTACTGTTGCC
ATCCGTATTGCTGGTTTCTCTGATTCTGCCCTCTTCCCGAGCTACCAGAGCCACCATACT
GACCACCTTGTGTGTAGTAGGGAGGTCC

Last Updated on 06.12.2018 16:11