Sequences from confirmation amplicon


Confirmation sequences for line 901F11 and hit At5g36680 are shown.
Line ID 901F11
Locus At5g36680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14406855
Primers used for PCR DA75 - 8474, show primer details
   
Sequence Name 78-K105821-0022-901-F11C-DA75
GenBank Accession LN513693 [GenBank]
Sequencing primer gene specific primer, DA75: 5'-ATTTGTTCAAGTAGCCAAAAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K105821-0022-901-F11C-DA75
GTTTGATTATCTTCTCTGGCTACTATACATCAGGTACAGAATTTCTTATACCAAATCATA
AGTTTTTTTCTTATACATATGAAGATAGAGCATAGACTAAGAGCATAATTTGGAATCAAC
AATTGAAAACAATGAATTATGCTTGACAATACTATTAGACGAATACTTTGTAATTATTTT
TGCAACTGCTAATTCATGTAAAAGGACAAGGAAACAAAAAACTGGTGGCTTACACAAACA
AATCCAGATGATGATACTAAAAATCATTTGGGATATTGGCCAAAGGAGTTGTTTAATCTT
CTAGGAGATGGTGCTGATTTCGTTGGATTTGGAGGAATGGTTTCAGGTGATCCAAGAACT
CCAAGCCCACCGATGGGTAATGGTCGTCTTCCAAACAAAGATGACCGTCTATGGTCTGGT
TATCTTGACCATCTTACCATAATCCAACCGGACTATGAACATGCCGGATTTAATTACCCT
ATGACAGAGCCTTTGGTGGACAGCAACGTATGCTATGATGTTAATTTAGTAGGATATGTT
GATCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGC
   
Sequence Name 86-K105822-0022-901-F11C-8409
GenBank Accession LN514657 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K105822-0022-901-F11C-8409
CTTTACAATTGAATATATCCTGATGATCAACATATCCTACTAAATTAACATCATAGCATA
CGTTGCTGTCCACCAAAGGCTCTGTCATAGGGTAATTAAATCCGGCATGTTCATAGTCCG
GTTGGATTATGGTAAGATGGTCAAGATAACCAGACCATAGACGGTCATCTTTGTTTGGAA
GACGACCATTACCCATCGGTGGGCTTGGAGTTCTTGGATCACCTGAAACCATTCCTCCAA
ATCCAACGAAATCAGCACCATCTCCTAGAAGATTAAACAACTCCTTTGGCCAATATCCCA
AATGATTTTTAGTATCATCATCTGGATTTGTTTGTGTAAGCCACCAGTTTTTTGTTTCCT
TGTCCTTTTACATGAATTAGCAGTTGCAAAAATAATTACAAAGTATTCGTCTAATAGTAT
TGTCAAGCATAATTCATTGTTTTCAATTGTTGATTCCAAATTATGCTCTTAGTCTATGCT
CTATCTTCATATGTATAAGAAAAAAACTTATGATTTGGTATAAGAAATTCTGTACCTGAT
GTATAGTAAGCCAGAGAAGATAATCAAACTTGAGAGGTACACTTATTGGACCATTTTTGG
CTACTGAAACAAA

Last Updated on 06.12.2018 16:11