Sequences from confirmation amplicon


Confirmation sequences for line 903C07 and hit At3g47000 are shown.
Line ID 903C07
Locus At3g47000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17315600
Primers used for PCR 23ow - 8474, show primer details
   
Sequence Name 50-K114864-0022-903-C07C-23ow
GenBank Accession LN507262 [GenBank]
Sequencing primer gene specific primer, 23ow: 5'-TGAGCAAGACACTTCAGATAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K114864-0022-903-C07C-23ow
GAGCGATGGTGTTCCCTTCATTTATGCCCTTGTCAGTACCACCATCTCCAACAAAGTGTT
TGACACACGCGACGACGTTGTTTCTATAACCCCAATAAACAAATCCATGAGAAATGACAT
CAAACCTATTAATGTCCCAAATAAGTTTACAGTGTACTGATTCATATGATACCTTCCTGC
AACAAAAGGATAACCATTTGGGTGTTCTTCTGGTGGCACACCTTGTAGGCCCGAGACAAG
AGAAGTCATCTCACAGACAAGTTCAGGATCTTCTCCATAACTCTCATAGCATCTTCCCCA
CCTCGGATCTCTCAAATCATTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 10-K114863-0022-903-C07C-8409
GenBank Accession LN490776 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114863-0022-903-C07C-8409
ATTTACAATTGAATATTGAATGATTTGAGAGATCCGAGGTGGGGAAGATGCTATGAGAGT
TATGGAGAAGATCCTGAACTTGTCTGTGAGATGACTTCTCTTGTCTCGGGCCTACAAGGT
GTGCCACCAGAAGAACACCCAAATGGTTATCCTTTTGTTGCAGGAAGGTATCATATGAAT
CAGTACACTGTAAACTTATTTGGGACATTAATAGGTTTGATGTCATTTCTCATGGATTTG
TTTATTGGGGTTATAGAAACAACGTCGTCGCGTGTGTCAAACACTTTGTTGGAGATGGTG
GTACTGACAAGGGCATAAATGAAGGGAACACCATCGCTTCATATGAAGAATTAGAGAAGA
TACATATTCCTCCTTATCTGAAGTGTCTTGCTC

Last Updated on 06.12.2018 16:11