Sequences from confirmation amplicon


Confirmation sequences for line 905B11 and hit At3g22850 are shown.
Line ID 905B11
Locus At3g22850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8089397
Primers used for PCR DA78 - 8474, show primer details
   
Sequence Name 15-K105749w-0022-905-B11C-DA78
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, DA78: 5'-TTACCATTTTGATCTGTTGCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K105749w-0022-905-B11C-DA78
TGTTGGCGATTTTCGACAGAACGTGGCGAAAACACCCGAGGCTCTTCAGGGTCAAGAGGG
TGGATCGGTTTGTGCTCTTAAAGATAGGTTCTTGCCGAACCATTTCTCCTCTGTTTATCC
TGGTGCTGTCACCATCAATCTCGGATCTTCTGGTTTCATTGCTTGCTCTCTCGAGAAACA
GAACCCTCTTCTTCCCAGGTTTTGTACAATAGTTTATTCCTCAGGATGATGTTTTCTTCT
TCTGTCCTAGATATGAGAGATTTGCTATCTTAATGTTTCACTGGCTTGCAAAGATAGTTT
AGGATATGTTTCACTGAATCTGAGAGATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 55-K105750-0022-905-B11C-8409
GenBank Accession LN508814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K105750-0022-905-B11C-8409
GCCATTTACATTGAATCTCTCAGATTCAGTGAAACATATCCTAAACTATCTTTGCAAGCC
AGTGAAACATTAAGATAGCAAATCTCTCATATCTAGGACAGAAGAAGAAAACATCATCCT
GAGGAATAAACTATTGTACAAAACCTGGGAAGAAGAGGGTTCTGTTTCTCGAGAGAGCAA
GCAATGAAACCAGAAGATCCGAGATTGATGGTGACAGCACCAGGATAAACAGAGGAGAAA
TGGTTCGGCAAGAACCTATCTTTAAGAGCACAAACCGATCCACCCTCTTGACCCTGAAGA
GCCTCGGGTGTTTTCGCCACGTTCTTGTCGAAAATCGCCAACATTTTCTTCTTCTTCCCG
ATCACGCACAGTGAAACAGAGCAACAGATCAAAATGGTA

Last Updated on 06.12.2018 16:11