Sequences from confirmation amplicon


Confirmation sequences for line 905D10 and hit At3g10060 are shown.
Line ID 905D10
Locus At3g10060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3103362
Primers used for PCR NK63 - 8474, show primer details
   
Sequence Name 40-K103460-0022-905-D10C-NK63
GenBank Accession LN503680 [GenBank]
Sequencing primer gene specific primer, NK63: 5'-AGGCAGTAAGCACCAGTAGAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K103460-0022-905-D10C-NK63
CATACATTGTGTTCTTGACTCAAAACTCAATCTCTTACATACAATTCTCTGCAGCTACGT
GCATCCAAGTTACCGGAAAGCGATTTCACGACTCTCCCCAATGGTCTCAAGTCCGTAATC
CAATGCATCATCTTTTGTTTGAATTCGATACAGTACTCATACCGCAAAACAGGATTGAAA
AATCAAGTCTTTCTCGCAATAATCACAAGTTTGGTTTCTGTTTATATTGTGGCAGGTACT
ATGATATAAAGGTTGGCAATGGAGCAGAGGCTGTGAAAGGATCTCGGGTCGCAGTACGGT
TGTGAATACGTTTCCTGGTTTACTTTGGACATGTAAATGCTAACAGTTTTAACAAGCGTT
GATGCTTAACCCAAATGTTGTGTCGCAGGTTCACTATGTTGCAAAATGGAAAGGGATAAC
GTTCATGACAAGTCGACAAGGACTTGGTGTTGGAGGTGGAACGGTATAAGGCAATCTTAC
AGAGTCCATTACTGTACTTGAATATGCAAGGATCCTTTACTGATAATAATGAATTTTTGC
AGCCTTATGGGTTTGACGTTGGTCAATCAGAGAGAGGCAATGTAAACCGAAGGGATGCGT
GTAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 49-K103461-0022-905-D10C-8409
GenBank Accession LN506809 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103461-0022-905-D10C-8409
TTACAATTGAACCATTACACGCATCCCTTCGGTTTACATTGCCTCTCTCTGATTGACCAA
CGTCAAACCCATAAGGCTGCAAAAATTCATTATTATCAGTAAAGGATCCTTGCATATTCA
AGTACAGTAATGGACTCTGTAAGATTGCCTTATACCGTTCCACCTCCAACACCAAGTCCT
TGTCGACTTGTCATGAACGTTATCCCTTTCCATTTTGCAACATAGTGAACCTGCGACACA
ACATTTGGGTTAAGCATCAACGCTTGTTAAAACTGTTAGCATTTACATGTCCAAAGTAAA
CCAGGAAACGTATTCACAACCGTACTGCGACCCGAGATCCTTTCACAGCCTCTGCTCCAT
TGCCAACCTTTATATCATAGTACCTGCCACAATATAAACAGAAACCAAACTTGTGATTAT
TGCGAGAAAGACTTGATTTTTCAATCCTGTTTTGCGGTATGAGTACTGTATCGAATTCAA
ACAAAAGATGATGCATTGGATTACGGACTTGAGACCATTGGGGAGAGTCGTGAAATCGCT
TTCCGGTAACTTGGATGCACGTAGCTGCAGAGAATTGTATGTAAGAGATTGAGTTTTGAG
TCAAGAACACAATGTATGCATAGATATATATATTGCTTACAGCTCTTCTACT
Primers used for PCR AE07 - 8474, show primer details
   
Sequence Name 36-K117317-0022-905-D10C-AE07
GenBank Accession LN502381 [GenBank]
Sequencing primer gene specific primer, AE07: 5'-GGCTTCATTAGTCCTTTTAGCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117317-0022-905-D10C-AE07
GAAGCTTTCGGGCACTTTTTTCGATCAAATTTACCCAGTGTATTAGTTGATTGATTTCAC
ATACCCGAAAGGACTCTGCTTGATTGATAACAGCTCAATGTCAAGCTGCAAAAGAAAGAT
AACATAAATGAACACCACTTTAGAAGCCAACAAAAATTTCAATCCATTCAACTTGGTTAC
CTCTATCGTAGCGTTTGGAGGAATCTCTTGCACTCCTTTCTTCCCGTAAGCCAGCTCGGG
AGGAACAATCACCAATCTCTGTATATAAACCTCATAGGGTCATCAAAACAAGACTTTGAG
TGGCCTGACTTTGTGAAGCCTTTCGGTTTCAAAAACTAACCTGACCGCCTACACGCATCC
CTTCAACACCAAACGAAATGATGTTGAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCACA
   
Sequence Name 27-K117316-0022-905-D10C-8409
GenBank Accession LN498570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117316-0022-905-D10C-8409
ATTTCACATCATTTCGTTTGGTGTTGAAGGGATGCGTGTAGGCGGTCAGGTTAGTTTTTG
AAACCGAAAGGCTTCACAAAGTCAGGCCACTCAAAGTCTTGTTTTGATGACCCTATGAGG
TTTATATACAGAGATTGGTGATTGTTCCTCCCGAGCTGGCTTACGGGAAGAAAGGAGTGC
AAGAGATTCCTCCAAACGCTACGATAGAGGTAACCAAGTTGAATGGATTGAAATTTTTGT
TGGCTTCTAAAGTGGTGTTCATTTATGTTATCTTTCTTTTGCAGCTTGACATTGAGCTGT
TATCAATCAAGCAGAGTCCTTTCGGGTATGTGAAATCAATCAACTAATACACTGGGTAAA
TTTGATCGAAAAAAGTGCCCTGAAAGCTTTCTTTTCGGTTTTATTCTGCAGGACGCCAGT
GAAGATAGTTGAAGGCTAAAAGGACAAATGAAGC
Primers used for PCR NK63 - 8474, show primer details
   
Sequence Name 21-K117356-0022-905-D10C-NK63
GenBank Accession LN495834 [GenBank]
Sequencing primer gene specific primer, NK63: 5'-AGGCAGTAAGCACCAGTAGAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117356-0022-905-D10C-NK63
TTGTGTTCTTGATCAAACTCAATCTATTACATACATTCTCTGCAGCTACGTGCATCCAAG
TTACCGGAAAGCGATTTCACGACTCTCCCCAATGGTCTCAAGTCCGTAATCCAATGCATC
ATCTTTTGTTTGAATTCGATACAGTACTCATACCGCAAAACAGGATTGAAAAATCAAGTC
TTTCTCGCAATAATCACAAGTTTGGTTTCTGTTTATATTGTGGCAGGTACTATGATATAA
AGGTTGGCAATGGAGCAGAGGCTGTGAAAGGATCTCGGGTCGCAGTACGGTTGTGAATAC
GTTTCCTGGTTTACTTTGGACATGTAAATGCTAACAGTTTTAACAAGCGTTGATGCTTAA
CCCAAATGTTGTGTCGCAGGTTCACTATGTTGCAAAATGGAAAGGGATAACGTTCATGAC
AAGTCGACAAGGACTTGGTGTTGGAGGTGGAACGGTATAAGGCAATCTTACAGAGTCCAT
TACTGTACTTGAATATGCAAGGATCCTTTACTGATAATAATGAATTTTTGCAGCCTTATG
GGTTTGACGTTGGTCAATCAGAGAGAGGCAATGTAAACCGAAGGGATGCGTGTAATGGCT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCA
   
Sequence Name 01-K117355-0022-905-D10C-8409
GenBank Accession LN486752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117355-0022-905-D10C-8409
ATTTACATTGAGCCATTACACGCATCCCTTCGGTTACATTGCCTCTCTCTGATTGACCAA
CGTCAAACCCATAAGGCTGCAAAAATTCATTATTATCAGTAAAGGATCCTTGCATATTCA
AGTACAGTAATGGACTCTGTAAGATTGCCTTATACCGTTCCACCTCCAACACCAAGTCCT
TGTCGACTTGTCATGAACGTTATCCCTTTCCATTTTGCAACATAGTGAACCTGCGACACA
ACATTTGGGTTAAGCATCAACGCTTGTTAAAACTGTTAGCATTTACATGTCCAAAGTAAA
CCAGGAAACGTATTCACAACCGTACTGCGACCCGAGATCCTTTCACAGCCTCTGCTCCAT
TGCCAACCTTTATATCATAGTACCTGCCACAATATAAACAGAAACCAAACTTGTGATTAT
TGCGAGAAAGACTTGATTTTTCAATCCTGTTTTGCGGTATGAGTACTGTATCGAATTCAA
ACAAAAGATGATGCATTGGATTACGGACTTGAGACCATTGGGGAGAGTCGTGAAATCGCT
TTCCGGTAACTTGGATGCACGTAGCTGCAGAGAATTGTATGTAAGAGATTGAGTTTTGAG
TCAAGAACACAATGTATGCATAGATATATATATTGCTTACAGCTCTTCTACTGG

Last Updated on 06.12.2018 16:11