Sequences from confirmation amplicon


Confirmation sequences for line 910H07 and hit At3g22425 are shown.
Line ID 910H07
Locus At3g22425 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7951301
Primers used for PCR 26jr - 8474, show primer details
   
Sequence Name 56-K113214-0022-910-H07C-8409
GenBank Accession LN509159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K113214-0022-910-H07C-8409
ATGAAGCCATTTACATTGAAGCCATGTAGATTTCTTCTTCTTCTTCTATGGCATTAGGTC
AGTTTTGGGTCTAGACATGGCTGAGATTAAGCTCATTTCATGATTGATATTATTTCTGTT
CACTCAAAAAGTCGTCGTTTTTAATCTCAATCAGGTAGAATTGGAGAAGTAAAGAGAGTA
ACAAAGGAAACGAATGTTTCAGTGAAGATTAATTTGGATGGTACTGGAGTTGCAGATAGT
TCTAGTGGAATTCCTTTCCTTGACCATATGTTAGATGTAAAGTCTTTGCCTTTAAACCTT
TTTGTCATGTATTTTTGATTTCTTCAGTTTCCATTGGAAGAGAGCTTAACACAGACTTTT
TAATTTAAGGATCTTGTTTTGATTCATTATTGGAATGTTGACTGATTGATGTTTTTATGG
TTTCACTCTTTGTCTGTCTTGGTTTAGCAACTTGCTTCGCATGGCTTGTTTGATGTGCAC
GTTAGAGCTACTGGTGATGTTCACATTGATGA
   
Sequence Name 56-K113215-0022-910-H07C-26jr
GenBank Accession LN509160 [GenBank]
Sequencing primer gene specific primer, 26jr: 5'-ATCATCAATGTGAACATCACCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K113215-0022-910-H07C-26jr
CAGCCATGCGAAGCAGTTGCTAAACCAAGACAGACAAAGAGTGAAACCATAAAAACATCA
CTCTAAAAACATTCCAATAATGAATCAAAACAAGATCCTTAAATTAAAAAGTCTGTGTTA
AGCTCTCTTCCAATGGAAACTGAAGAAATCAAAAATACATGACAAAAAGGTTTAAAGGCA
AAGACTTTACATCTAACATATGGTCAAGGAAAGGAATTCCACTAGAACTATCTGCAACTC
CAGTACCATCCAAATTAATCTTCACTGAAACATTCGTTTCCTTTGTTACTCTCTTTACTT
CTCCAATTCTACCTGATTGAGATTAAAAACGACGACTTTTTGAGTGAACAGAAATAATAT
CAATCATGAAATGAGCTTAATCTCAGCCATGTCTAGACCCAAAACTGACCTAATGCCATA
GAAGAAGAAGAAGAAATCTACATGGCTTCAATTGTAAATGGCTTCATGTAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
A

Last Updated on 06.12.2018 16:11