Sequences from confirmation amplicon


Confirmation sequences for line 913F01 and hit At2g35890 are shown.
Line ID 913F01
Locus At2g35890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15067325
Primers used for PCR IF70 - 8474, show primer details
   
Sequence Name 93-K101387-0022-913-F01C-8409
GenBank Accession LN515241 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K101387-0022-913-F01C-8409
ATTTACATTGAATATATCCTGAAACCGAAGACACAGTTTTTCTTACATACTGGGATCATT
TTTAGACGGATGTGCACTTGGAAGACACAGTAGACTACAACGAATTCATAGAGGCGATTG
TAAGACTGAGACAAATACAAGAAGAGGAAGCAAATGATCGTTTAGAATCATCAACAAAAG
TGTAATGAAGTTGGCGTGAAAGATATTGTTGTTGTCTGAAGAAGTGTAATGAGTACTCAA
AACTGATGATGACGAATGTAAGTAAATGTGATGCATTCACTCACTTTAGTCTCTTAGAGC
TGTTGTTATAAAAGTCTCCTAATCAAAGATCTAGTGTATTTCAACATTTCANAAAACATA
ATCTATATATAATCTTTATCTTGTTACACTTTAGACTGTAGGAAACTANCAAAAGAGCCA
CAAACAATGACTA
   
Sequence Name 93-K101393-0022-913-F01C-IF70
GenBank Accession LN515242 [GenBank]
Sequencing primer gene specific primer, IF70: 5'-ACAAAGGCAAAGTATTGAGAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K101393-0022-913-F01C-IF70
CTTTCACTAAACTTCTATATACAACAAAACACATCTTTCATTTTTTTTTTTGCTCTAACT
TTTATAAGAACATGATAAACAAAACAAAAAAAAACCTACCCATCAAAATCATTTGACTTT
CCATCTATCAACTTATCCATCAACTTACTTTCTGGAGGTCCCTTTGTGTCCATCTTCCCC
TTGTTCTGCCCCCCCTCTAAAAGTCATTTGTTGGGGCTCTTTTTGTAGTTCCCTACAGTC
TAAAGTGTAACAAGATAAAGATTATATATGGATTATGTTTTTTGAAATGTTGAAATACAC
TAAATCTTTGATTAGGAGACTTTTATAACAACAGCTCTAAAAGACTAAAGTGAGTGAATG
CATCACATTTACTTACATTCGTCATCATCAGTTTTGAGTACTCATTACACTTC

Last Updated on 06.12.2018 16:11