Sequences from confirmation amplicon


Confirmation sequences for line 915D11 and hit At1g66840 are shown.
Line ID 915D11
Locus At1g66840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24934148
Primers used for PCR 29wm - 8474, show primer details
   
Sequence Name 60-K119006-0022-915-D11C-29wm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 29wm: 5'-CTGGTCATGTATGAAATTTGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K119006-0022-915-D11C-29wm
ACATTCATTACAAAATATATATGAAAAATTTACCTGACTCTTATACAATGCAATACATTA
TTTTCATTAATATACACCTAAANGTCTACATAAATACATCATTTTTTTATTATCAAAAAA
TAACAAAAATACAAAACAAATAAATCACTTACAAGCTAAAAACAATGTGAAACTTAACAC
GCAAGCAATAAAAAAACTTACACTCACTTCAACAAGATAACTAAACTTCTCATTGTTCTA
AAGAAGAATTACGCCTCTTCCGGCTAAAAAATTTGACCATGTTTGGAACTTTCTTCTTCT
TCTTGATTACAAAGAATGTTGGAGTCGCTCGGTTCCCTGATGAGTACCTTCTCGACTTCC
CTCCCTGAACCGGTGCAAATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 30-K119005-0022-915-D11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K119005-0022-915-D11C-8409
CATTTCAATTGAATATATTCATTTGCACCGGTTCAGGGAGGGAAGTCGAGAAGGTACTCA
TCAGGGAACCGAGCGACTCCAACATTCTTTGTAATCAAGAAGAAGAAGAAAGTTCCAAAC
ATGGTCAAATTTTTTAGCCGGAAGAGGCGTAATTCTTCTTTAGAACAATGAGAAGTTTAG
TTATCTTGTTGAAGTGAGTGTAAGTTTTTTTATTGCTTGCGTGTTAAGTTTCACATTGTT
TTTAGCTTGTAAGTGATTTATTTGTTTTGTATTTTTGTTATTTTTTGATAATAAAAAAAT
GATGTATTTATGTAGACTTTAGGTGTATATTAATGAAAATAATGTATTGCATTGTATAAG
AGTCAGGTAAATTTTTTCATATTTGATTTTGGTAATTTGAATGGTTTATATCGAACTAGA
ACCAACAAATTTCATACATGACCA

Last Updated on 06.12.2018 16:11