Sequences from confirmation amplicon


Confirmation sequences for line 915E07 and hit At1g05250 are shown.
Line ID 915E07
Locus At1g05250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1525619
Primers used for PCR 16yi - 8474, show primer details
   
Sequence Name 83-K120200-0022-915-E07C-16yi
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 16yi: 5'-TCAATCTTGATTCTTACCCCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K120200-0022-915-E07C-16yi
AAACCACATTGTCTTCTTTGATGTTAGTAATGTATACACTCTCTTATTGCCAGTGGTCGT
TGACCATTAGTAAAGCCATGGGTATTGTTATAATATGGAAGGCGAATTTAGATCTAAAGA
GCTCATCCAACATAAAGAAGAGAGATTTAAAAGAACAAATTGCAAGTATCATTTGAAGCA
GAGACAATGAGAAGAGCTCCATCCCTAAACAACAACCGTGTCAAGCATGGTGCCTAATGT
CTGAACTATGTTTGATGACATATGCTCAATCGTAGTCATTCACCTTTTGAATTTTGTCTT
TTCTCATATACCGCACAAGTAGATTCCAATATCTAACCAACATCCAGTCACAATTTTGAA
TACGACAAATCAACGAAGCCCCACATAAACAAACAATCAAAGAAATTTTAATCTCATAAT
AAAGGTAAACGACAGTAATGGTACACGTATTATTAGCTAGTACAGTGAACAACAAATCAA
CACAATAATGTTGAACTAGTTCGTTGCATCAGGAACATCATAGTAGCGTATAATAAGTGT
GTTTTGTTACCGGGCCTAAGGTGTAAGGTCTTGTATGAAAGTTCTGTTTTCTTGATGGAT
ATAGTCCAGAGAAGATCCGAATCCGATTCGACTGTGATTTTCTCTTCTTTAACCGTTTTA
ACTATGAATTAATATTTTTGTTATTGATGGCCTTAACTTTAGTATGTTTTACTTCGGCCT
TAACATTCATCAATAACAAAAATAAAACACACCTATGATAAGGCCAATGTTAAGGGCAAA
GTAAAACATACGAAATTTAATATGTAAGGCCAAAGTCCAATGTTATCATATGTAAGGCCA
AAGTCCAATGTGTTTGTTTCCCACCTATGATCCTAAAATATCAATAAAAATGAAAACTCC
TTGACATAAAAACATAAATAAATTTTTTAA
   
Sequence Name 65-K120199-0022-915-E07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K120199-0022-915-E07C-8409
ATATTTATGTTTGTTTTTCACGGTTAAAAATTTATTTATGTTTTTATGTCAAGGAGTTTT
CATTTTTATTGATAATTTTAGGATCATAGGTGGGAAACAAACACATTGGACTTTGGCCTT
ACATATGATAACATTGGACTTTGGCCTTACATATTAAATTTCGTATGTTTTACTTTGGCC
TTAACATTGGCCTTATCATAGGTGTGTTTTATTTTTGTTATTGATGAATGTTAAGGCCGA
AGTAAAACATACTAAAGTTAAGGCCATCAATAACAAAAATATTAATTCATAGTTAAAACG
GTTAAAGAAGAGAAAATCACAGTCGAATCGGATTCGGATCTTCTCTGGACTATATCCATC
AAGAAAACAGAACTTTCATACAAGACCTTACACCTTAGGCCCGGTAACAAAACACACTTA
TTATACGCTACTATGATGTTCCTGATGCAACGAACTAGTTCAACATTATTGTGTTGATTT
GTTGTTCACTGTACTAGCTAATAATACGTGTACCATTACTGTCGTTTACCTTTATTATGA
GATTAAAATTTCTTTGATTGTTTGTTTATGTGGGGCTTCGTTGATTTGTCGTATTCAAAA
TTGTGACTGGATGTTGGTTAGATATTGGAATCTACTTGTGCGGTATATGAGAAAAGACAA
AATTCAAAAGGTGAATGACTACGATTGAGCATATGTCATCAAACATAGTTCAGACATTAG
GCACCATGCTTGACACGGTTGTTGTTTAGGGATGGAGCTCTTCTCATTGTCTCTGCTTCA
ATGATACTTGCAATTTGTTCTTTTAAATCTCTCTTCTTTATGTTGGATGAGCTCTTTAGA
TCTAAATTCGCCTTCCATATTATAACAATACCCATGGCTTTA

Last Updated on 06.12.2018 16:11