Sequences from confirmation amplicon


Confirmation sequences for line 915G03 and hit At2g22980 are shown.
Line ID 915G03
Locus At2g22980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9783033
Primers used for PCR 20nw - 8474, show primer details
   
Sequence Name 56-K114664-0022-915-G03C-20nw
GenBank Accession LN509187 [GenBank]
Sequencing primer gene specific primer, 20nw: 5'-CGACTCCTACCAACTAGGTTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114664-0022-915-G03C-20nw
GTGACCATGATATGATGGTGCCTTTCCTTGCAACTCAAGCCCGCTAAAGATCTCTCAATT
ATTCCATCACTGATGATTGGAAGCCTTGGATGATAAACGATCAAATTGCTGGGTATATAT
ATGTTTTTCTGTTTCTCTTGTTCATTCATTTTCTAGTCGTATCACACACTTATCAAACTC
ACATAAAAATCATTCTTGCCTTTGTTTCTTGACAGATACACGAGAAGTTATTCCAATAAG
ATGACATTTGCTACTATCAAAGCAAGTCTTCTTGTGTCAACTTAATATTTGTATTGTCCT
TAGACTGCAATAAAAAAGACTTTGTTTTAATTGTTTTGAGTTTGTTTTATTTATTTTTTT
GTTAAAGGGAAGTGGACACACAGCAGAGTATAAACCAAAAGAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 27-K114663-0022-915-G03C-8409
GenBank Accession LN498507 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114663-0022-915-G03C-8409
ATATATCCTCTTTTGGGTTTATACTCTGCTGTGTGTCCTCTACCCCTTTAACAAAAAAAT
AAATAAAACAAACTCAAAACAATTAAAACAAAGTCTTTTTTATTGCAGTCTAAGGACAAT
ACAAATATTAAGTTGACACAAGAAGACTTGCTTTGATAGTAGCAAATGTCATCTTATTGG
AATAACTTCTCGTGTATCTGTCAAGAAACAAAGGCAAGAATGATTTTTATGTGAGTTTGA
TAAGTGTGTGATACGACTAGAAAATGAATGAACAAGAGAAACAGAAAAACATATATATAC
CCAGCAATTTGATCGTTTATCATCCAAGGCTTCCAATCATCAGTGATGGAATAATTGAGA
GATCTTATCCAGGCTTGAGTTGCAAGGAAAGGCACCATCATATCATGGTCACCACTGTTC
ACCATAAACCTCACAAATGTTATAATCTCTTGAACCTAGTTGTAAAGGAGTCG

Last Updated on 06.12.2018 16:11