Sequences from confirmation amplicon


Confirmation sequences for line 915H05 and hit At2g35100 are shown.
Line ID 915H05
Locus At2g35100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14794662
Primers used for PCR 70sm - 8474, show primer details
   
Sequence Name 27-K116390-0022-915-H05C-70sm
GenBank Accession LN498550 [GenBank]
Sequencing primer gene specific primer, 70sm: 5'-GATCTCTTCTACGTCCCTGTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116390-0022-915-H05C-70sm
GGACCGGTTACGCGATGAGAAGAGCAGGAAGGGTTGGTGGAGTGGCTTGAAGGTCAAGAG
TGGTGGAGGAGGAATGCTGGGAGAGATCACGTGATACCCGCAGGCGATCCGAATGCGTTG
TATCGGATCTTGGATCGGGTCAAAAACGCAGTTCTGCTGGTTTCGGATTTCGGGCGGTTG
AGGCCTGATCAAGGATCGTTCGTCAAGGATGTTGTAATACCTTACTCCCATAGAGTCAAT
CTCTTCAATGGAGAAATTGGAGTTGAGGATCGCAACACATTACTCTTCTTCATGGGAAAT
CGATATCGCAAAGATGTAAGTTGCTTAAAATGTTCCAATTCTGTAGCTTTCTATTATTAA
TGGCAAATGTACAGAAATGTGTATATAATCCATTGTGGTAATGCTATTATTATTAGTATT
GGCAAATATTCCATATACATACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K116389-0022-915-H05C-8409
GenBank Accession LN487189 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116389-0022-915-H05C-8409
ATCCTGTATGTATATGGAATATTTGCCAATACTAATAATAATAGCATTACCACAATGGAT
TATATACACATTTCTGTACATTTGCCATTAATAATAGAAAGCTACAGAATTGGAACATTT
TAAGCAACTTACATCTTTGCGATATCGATTTCCCATGAAGAAGAGTAATGTGTTGCGATC
CTCAACTCCAATTTCTCCATTGAAGAGATTGACTCTATGGGAGTAAGGTATTACAACATC
CTTGACGAACGATCCTTGATCAGGCCTCAACCGCCCGAAATCCGAAACCAGCAGAACTGC
GTTTTTGACCCGATCCAAGATCCGATACAACGCATTCGGATCGCCTGCGGGTATCACGTG
ATCTCTCCCAGCATTCCTCCTCCACCACTCTTGACCTTCAAGCCACTCCACCAACCCTTC
CTGCATCTTCTCATCGCTGTAACCGGATCCAGCTTCAACGGGTCGACCCGCATTTACAAT
TAAGCTGAGTGAAGAGAACACAGGGACGT

Last Updated on 06.12.2018 16:11