Sequences from confirmation amplicon


Confirmation sequences for line 917G02 and hit At4g09610 are shown.
Line ID 917G02
Locus At4g09610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6075451
Primers used for PCR 15hk - 8474, show primer details
   
Sequence Name 40-K112562-0022-917-G02C-15hk
GenBank Accession LN503757 [GenBank]
Sequencing primer gene specific primer, 15hk: 5'-TAATCCGTTTAAACCCATCAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112562-0022-917-G02C-15hk
ATCAAGCTTTAGTTCATAGTTTCCTTCTCCGAGATATAACATCTCATTAAAAGCTTGAGA
CATTCTCCATAACAAAGATATATATAAGTTGGGAAGAGAGTGCACCCATCAGAAATATTA
AGTTATATACCAACGTGTTATATATAAGTTAGGGTTACATACCGATTTTCACTACGCCTT
CACCGACCTTTGCACCATCAGCAGCGTGGACGTGAAGCTGAAGAGAATGATCAGTCATTA
TCATATAAACATGAAAGTTTAACATTATAAAATGGCTTAATTTAACACTTTACATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACGATTATA
   
Sequence Name 16-K112561-0022-917-G02C-8409
GenBank Accession LN493494 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K112561-0022-917-G02C-8409
CATTTACATTGATATATGTAAAGTGTTAAATTAAGCCATTTTATAATGTTAAACTTTCAT
GTTTATATGATAATGACTGATCATTCTCTTCAGCTTCACGTCCACGCTGCTGATGGTGCA
AAGGTCGGTGAAGGCGTAGTGAAAATCGGTATGTAACCCTAACTTATATATAACACGTTG
GTATATAACTTAATATTTCTGATGGGTGCACTCTCTTCCCAACTTATATATATCTTTGTT
ATGGAGAATGTCTCAAGCTTTTAATGAGATGTTATATCTCGGAGAAGGAAACTATGAACT
AAAAGCTTTGGATTCCTTTGCAACAAATATAAACTTTTGATGGGTTAAAACGGATT

Last Updated on 06.12.2018 16:11