Sequences from confirmation amplicon


Confirmation sequences for line 918E07 and hit At3g22880 are shown.
Line ID 918E07
Locus At3g22880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8098924
Primers used for PCR 86ra - 8474, show primer details
   
Sequence Name 43-K116132-0022-918-E07C-86ra
GenBank Accession LN504959 [GenBank]
Sequencing primer gene specific primer, 86ra: 5'-CTTGTTTACATTTCAGAGGCATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K116132-0022-918-E07C-86ra
ACACGAAAGGAGGGAACGGAAAAGTCGGCATACATTCGACACAGAGGGAACCTCGTATCC
TTAATATCCTTATAACAAATTCTTTCTGAACCAGAATCTTGCGTCTTCATTAGATTCGTT
TTCTATATTTCCATCGATTTTTTCCTTAACCCCTAAATTTTGTAGCCGTCCTGATAGGAT
TCGTCCCAATTCGCTGAAAGATTTGGAATGGATCCAGGAGCTCGTGCTTGACAATGTAAG
CATTATTAACCTCACATCTAAATCGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTTCAATT
   
Sequence Name 18-K116131-0022-918-E07C-8409
GenBank Accession LN494454 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K116131-0022-918-E07C-8409
ACGGCTACAAAATTTAGGGGTTAAGGAAAAATCATGGAATATAGAAACAATCTAATGAAG
ACACAAGATCTGGTCAGAAGATTTGTTATAAGATATTAAGGATACAAGGTTCCCTCTGTG
TCAATGTATGCCACTTTTCCATTCCCTCCTTTCATGTTTGTAGGTAGCTGTAATACATCA
ATACAGAAAATGAATACCAATGACATTGAGAGACAGAGTACCTCAATGGTTTATATGCTG
AAAGAAAGTAAAATCTCATCTCATGCCTCT

Last Updated on 06.12.2018 16:11