Genotyping and primer details


Genotyping and primer details for 918E07 - At3g22880
Line ID 918E07
Locus At3g22880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8098924 of line 918E07 Chr3:8098924
   
Insertion allele amplicon 8474 - 86ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 86ra: 5'-CTTGTTTACATTTCAGAGGCATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8099207
   
wt amplicon (over InsPos) 46si - 86ra
wt amplicon start Chr3:8098497
wt amplicon end Chr3:8099207
wt amplicon size 711 bp
Locus specific primer 86ra: 5'-CTTGTTTACATTTCAGAGGCATGA-3'
2nd locus specific primer 46si: 5'-AATGTAAGTAATGTTGTGGGAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11