Sequences from confirmation amplicon


Confirmation sequences for line 919C07 and hit At5g04347 are shown.
Line ID 919C07
Locus At5g04347 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1220162
Primers used for PCR 86gp - 8474, show primer details
   
Sequence Name 44-K116132-0022-919-C07C-86gp
GenBank Accession LN505297 [GenBank]
Sequencing primer gene specific primer, 86gp: 5'-TGAAACAGTGTTGAATCCAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116132-0022-919-C07C-86gp
CGTTTTTAGTTGGATTGTAAATTTGAAAACATTCAAAACTTTTGCATAAAACTAGGCTAC
ATATTCAATTATTATTATTTCTTATCACAGACAGAATAATGAAGTACGGATTGTAACATA
AGCTCCACCGCCGAGCGTTACACACTTCTTACGCACACATTAGAGTCAGATTTCTACAGT
GACCAATTTTATTCAAATTATAAAGAAAAGAAAAAAAACAAAGAAATGATCTTCATCAGA
CACACATACGTGAACTTCACGAATAATATGAAACCCTAATCCTTGGGCGTTTAAGCCTTT
GGCCAATCATATTTCTTTTTAGGAGGTGTCTCGGGATGTTGTGTGTAATAGATCCCATCC
TCTCTTGCTATCCACAAGTACCTAGGCCCAATATCCGCTTTCCATTGAGATCTGTATGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATT
   
Sequence Name 19-K116131-0022-919-C07C-8409
GenBank Accession LN494909 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116131-0022-919-C07C-8409
GCGGATCTTGGGCCTCGGTACTTGCGGATCGCAAGAGAGGAAGGGATCTATTACACACAA
CATCCCGAGACACCTCCTAAAAAGAAATATGATTGGCCAAAGGCTTAAACGCCCAAGGAT
TAGGGTTTCATATTATTCGTGAAGTTCACGTATGTGTGTCTGATGAAGATCATTTCTTTG
TTTTTTTTCTTTTCTTTATAATTTGAATAAAATTGGTCACTGTAGAAATCTGACTCTAAT
GTGTGCGTAAGAAGTGTGTAACGCTCGGCGGTGGAGCTTATGTTACAATCCGTACTTCAT
TATTCTGTCTGTGATAAGAAATAATAATAATTGAATATGTAGCCTAGTTTTATGCAAAAG
TTTTGAATGTTTTCAATTTACAATCCAACTAAAAACGGAACAACATCTCAAACAATTTAC
AATAAAATCAAAGATTTATATTCTTTATCTCAGATTACGTTTTAATTTCATAAAAAGTAA
CATAAGAAATTATACCTTTTTGGATC

Last Updated on 06.12.2018 16:11