Sequences from confirmation amplicon


Confirmation sequences for line 920C09 and hit At5g26670 are shown.
Line ID 920C09
Locus At5g26670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9318773
Primers used for PCR 36kq - 8474, show primer details
   
Sequence Name 71-K113756-0022-920-C09C-36kq
GenBank Accession LN512594 [GenBank]
Sequencing primer gene specific primer, 36kq: 5'-AGACAAAGACCTGGATTTTCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K113756-0022-920-C09C-36kq
TTTGTTTCTCATATAGTTAGAAGATCCGCGACGCGACTTCTTTCGGTATACACAGTTCCT
AATGTTGTCACACCATCCTCCTCCCTTTCCACATTTCCAATTTCAACACACCATTCAAAA
AAATTTTAGTAACAAACATTACTAAAAACAGAAACTTCTTTCGCTAACCTCTAATTGAAT
TAACCAGCTATTGGCTCCAGATCCATGTCCACGATGTAAGTGATATCCAGGCAATGTACC
ATCCAAACAAACTAGTAATTAAGACACATAAGACTTAGATTCAAACACTGTTCTGTTTTT
ACATTTCAGTTTCACATAACACAATCAGAAAGTAAGTTACTTTTAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATA
   
Sequence Name 33-K113755-0022-920-C09C-8409
GenBank Accession LN501075 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K113755-0022-920-C09C-8409
AGTACTTACTTTCTGATTGTGTTATGTGAAACTGAAATGTAACAATATAACAGTGTTTGA
ATCTAAGTCTTATGTGTCTTAATTACTAGTTTGTTTGGATGGTACATTGCCTGGATATCA
CTTACATCGTGGACATGGATCTGGAGCCAATAGCTGGTTAATTCAATTAGAGGTTAGCGA
AAGAAGTTTCTGTTTTTAGTAATGTTTGTTACTAAAATTTTTTTGAATGGTGTGTTGAAA
TTGGAAATGTGGAAAGGGAGGAGGATGGTGTGACAACATTAGGAACTGTGTATACCGAAA
GAAGTCGCGTCGCGGATCTTCTAACTATATGGAGAAACAAATACAGTTTACAGGAATACT
TAGCAATAAAGCTCAAGAAAATCCAGGTCTTTGTCTA

Last Updated on 06.12.2018 16:11