Sequences from confirmation amplicon


Confirmation sequences for line 920E04 and hit At1g09640 are shown.
Line ID 920E04
Locus At1g09640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3121878
Primers used for PCR 43uj - 8474, show primer details
   
Sequence Name 54-K117387-0022-920-E04C-43uj
GenBank Accession LN508606 [GenBank]
Sequencing primer gene specific primer, 43uj: 5'-TCAGCTTTGCTATCAGGGAATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K117387-0022-920-E04C-43uj
TCTATATTGTGGCTCAATGCAGGATTCTGGGACATGTATGACCCAGAGGGATACTCACTA
TGGTTCTGTGACTACAAGTACAACGATGAGAACATGGTGTCATTTGTCACGCTCAACAAG
GTTGGTGGATCACCAATTTTTAATCAATTCAGTGATAGTTTAAATTGCATTTATCATTGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGAGTTATTAT
   
Sequence Name 27-K117386-0022-920-E04C-8409
GenBank Accession LN498573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117386-0022-920-E04C-8409
TTGAATATATCCATGATAAATGCAATTTAAACTATCACTGAATTGATTAAAAATTGGTGA
TCCACCAACCTTGTTGAGCGTGACAAATGACACCATGTTCTCATCGTTGTACTTGTAGTC
ACAGAACCATAGTGAGTATCCCTCTGGGTCATACATGTCCCAGAATCCTGCATTTGAGCC
ACAATATTAGACATGATAAAAAACAATTGATTCCAATAGCTAAATAATTCCCTGATAGCC
AAAGCTGA

Last Updated on 06.12.2018 16:11