Sequences from confirmation amplicon


Confirmation sequences for line 923A11 and hit At3g08950 are shown.
Line ID 923A11
Locus At3g08950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2728808
Primers used for PCR AU20 - 8474, show primer details
   
Sequence Name 45-K106289-0022-923-A11C-AU20
GenBank Accession LN505539 [GenBank]
Sequencing primer gene specific primer, AU20: 5'-GCTTCCAAAAATGGGAATAATGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K106289-0022-923-A11C-AU20
GATCTCTTTTACAACGCCGTCTGTCAACGAGTCAACATCGTGATTCTTCCCGTAGAATTT
CACAAAATTCATCTCGCGGACTCATCAAGTACCTTCACCCACAACATGTTTACATATCAA
AACTCCAACCACCAAAAAAGAGATCAAACACTACTGAACACATACAAGATGAATCATTAC
ACTGTAACTATGTAAAATGTAACTATGTAAATTCTGAAATTTGAGAATATGAACATGAAC
AATTCTAAGTCTGCATGTCGGGTTTTGTGCCGATAACTATAATCTTCCTAACAAAATATG
TTAAAGGGATAATAAGTACACGTACATGACTATAGAGTGATCAACGAGATAGTCTGAATC
TTCCTCTTCGGTCTTCATGTAGTAAACCCGGTAAGATCGGGCCACCGATTTGATTTCTTC
AGGGCTCCCAGTTAACCCGATCAATTTCGGGTGAAATTCTACATGTCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
ACGGTTATT
Primers used for PCR 09ax - 8474, show primer details
   
Sequence Name 89-K118414-0022-923-A11C-09ax
GenBank Accession LN514984 [GenBank]
Sequencing primer gene specific primer, 09ax: 5'-CTGCCATTGACAAAATAAGTGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K118414-0022-923-A11C-09ax
TTGGAGATTTTTCCTCTAAGGTTATATCACATTTACACAGAGGAAAATTCGGGAGTAGAC
GTGGTGCCAGTGTTTATCTCTGTGGACCCTGAAAGAGACACAGTTCAGCAAGTACATGAA
TATGTCAAAGGTAGATATTTTTCATTTCGAAAGATTCATTTGGTTTTGAAATGAAAATTC
ATAGTCTGATACTTCAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 41-K118413-0022-923-A11C-8409
GenBank Accession LN504256 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118413-0022-923-A11C-8409
TTTAAATTGAAATATTGAAGTATCAGACTATGAATTTTCATTTCAAAACCAAATGAATCT
TTCGAAATGAAAAATATCTACCTTTGACATATTCATGTACTTGCTGAACTGTGTCTCTTT
CAGGGTCCACAGAGATAAACACTGGCACCACGTCTACTCCCGAATTTTCCTCTGTGTAAA
TGTGATATAACCTTAGAGGAAAAATCTCCAAATGTAGAATCACACAAATGAAAAAATGTT
GGTATCTCACTTATTTTGTCAATGGCA
Primers used for PCR 77wl - 8474, show primer details
   
Sequence Name 90-K118414-0022-923-A11C-77wl
GenBank Accession LN515068 [GenBank]
Sequencing primer gene specific primer, 77wl: 5'-TGTCGTACATTTTGCTAATGGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K118414-0022-923-A11C-77wl
CTTATGATCACAGGCCTTAGACCTTAGCAAACGATATCTGATTTGGCTAGTGATAACCTT
GGCAAGATAAATGAACTTATCACTTCTATCACTGAGTAAATGCTTAGGGATTTCACATTG
ATTTCTGCTGAATCCCTCTTCGAGCTTATCTCGATTCTTCTTTGTATATTGACTTGGGTT
CTTATTGATTTAGATATAAATAAGAACTCTATAGCCGTCAAGGAGGGGCCATCTGCTGGA
AAAGCAGCCATTGGAGGACCATTCAGCCTTATAAGAGACGATGGGAAACGTGTGACGGAG
AAAAACTTGATGGGAAAATGGACCATTTTATACTTTGGTTTCACTCATTGTCCCGATATC
TGTCCTGACGAACTTATTAAGTTAGCTGCTGCCATTGACAAAATAAGTGAGATACCAACA
TTTTTTCATTTGTGTGATTCTACATTTGGAGATTTTTCCTCTAAGGTTATATCACATTTA
CACAGAGGAAAATTCGGGAGTAGACGTGGTGCCAGTGTTTATCTCTGTGGACCCTGAAAG
AGACACAGTTCAGCAAGTACATGAATATGTCAAAGGTAGATATTTTTCATTTCGAAAGAT
TCATTTGGTTTTGAAATGAAAATTCATAGTCTGATACTTCAATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 42-K118413-0022-923-A11C-oriL
GenBank Accession LN504636 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118413-0022-923-A11C-oriL
ATTTACATTGAATATTTGAAGTATCAGACTATGAATTTTCATTTCAAAACCAAATGAATC
TTTCGAAATGAAAAATATCTACCTTTGACATATTCATGTACTTGCTGAACTGTGTCTCTT
TCAGGGTCCACAGAGATAAACACTGGCACCACGTCTACTCCCGAATTTTCCTCTGTGTAA
ATGTGATATAACCTTAGAGGAAAAATCTCCAAATGTAGAATCACACAAATGAAAAAATGT
TGGTATCTCACTTATTTTGTCAATGGCAGCAGCTAACTTAATAAGTTCGTCAGGACAGAT
ATCGGGACAATGAGTGAAACCAAAGTATAAAATGGTCCATTTTCCCATCAAGTTTTTCTC
CGTCACACGTTTCCCATCGTCTCTTATAAGGCTGAATGGTCCTCCAATGGCTGCTTTTCC
AGCAGATGGCCCCTCCTTGACGGCTATAGAGTTCTTATTTATATCTAAATCAATAAGAAC
CCAAGTCAATATACAAAGAAGAATCGAGATAAGCTCGAAGAGGGATTCAGCAGAAATCAA
TGTGAAATCCCTAAGCATTTACTCAGTGATAGAAGTGATAAGTTCATTTATCTTGCCAAG
GTTATCACTAGCCAAATCAGATATCGTTTGCTAAGGTCTAAGGCCTGTGATCATTAAGCT
ACAAACAATGTATTCAATTAGACATTTCAGAACTCCATTAGC

Last Updated on 06.12.2018 16:11