Sequences from confirmation amplicon


Confirmation sequences for line 923C11 and hit At5g52540 are shown.
Line ID 923C11
Locus At5g52540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21323510
Primers used for PCR 19mh - 8474, show primer details
   
Sequence Name 56-K114180-0022-923-C11C-19mh
GenBank Accession LN509175 [GenBank]
Sequencing primer gene specific primer, 19mh: 5'-TATGAAATAGCAAAGGCTCAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114180-0022-923-C11C-19mh
CCATAGGAATAGTGTGAATGTTATGATCCTTATACCTGATCCAATCAAAAAGGCCAAGAG
AAGCTTTCCAGTGGACTGAACCACACGACGCAAATCAGCTCTAAACAACAACAGAGGAAC
AGCTAATGGAAGCAGAAAATTCAACACAACAGCAAAAGCAGGAGCTTGAGAAGAAATGAT
CCCTAGATTACTAGCTGCAAGACCAACTAGAGTGCTGACTAACGCACCACTCATAGCGGC
TCCGACCTTTGTCTTCTCCGACCTATACAATCAATCCCACACCATTTCCGATTTCAAACT
TCACAATTGGTATCACACAAAATCAACTTCACTTAAGAATCAACACAACGTAAAATTCAA
ATCTCCGATCAGATAAAGTAACGATCGAAGTTCTATAAAGAGTGATGATCGTACCAGAGA
CCGAGAGCACCAGTAGCGAAGAGAGCAGTCCAAGTTCCCCATTCATCGTTGGGAGAAATG
AGAGGCGTACTCAACGAACTCACAGTAACCGATCGGATCGGAGATGATGATTCCGGCGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 20-K114177-0022-923-C11C-8409
GenBank Accession LN495303 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114177-0022-923-C11C-8409
ATCGCCGGAATCATCATCTCCGATCCGATCGGTTACTGTGAGTTCGTTGAGTACGCCTCT
CATTTCTCCCAACGATGAATGGGGAACTTGGACTGCTCTCTTCGCTACTGGTGCTCTCGG
TCTCTGGTACGATCATCACTCTTTATAGAACTTCGATCGTTACTTTATCTGATCGGAGAT
TTGAATTTTACGTTGTGTTGATTCTTAAGTGAAGTTGATTTTGTGTGATACCAATTGTGA
AGTTTGAAATCGGAAATGGTGTGGGATTGATTGTATAGGTCGGAGAAGACAAAGGTCGGA
GCCGCTATGAGTGGTGCGTTAGTCAGCACTCTAGTTGGTCTTGCAGCTAGTAATCTAGGG
ATCATTTCTTCTCAAGCTCCTGCTTTTGCTGTTGTGTTGAATTTTCTGCTTCCATTAGCT
GTTCCTCTGTTGTTGTTTAGAGCTGATTTGCGTCGTGTGGTTCAGTCCACTGGAAAGCTT
CTCTTGGCCTTTTTGATTGGATCAGGTATAAGGATCATAACATTCACACTATTCCTATGG
TTATGCTCTTGATATTTCTGACTTTGAATTATCAGAAAAACCTGAGCCTTTGCTATTTCA
T

Last Updated on 06.12.2018 16:11