Sequences from confirmation amplicon


Confirmation sequences for line 924D03 and hit At1g77120 are shown.
Line ID 924D03
Locus At1g77120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28976616
Primers used for PCR BU45 - 8474, show primer details
   
Sequence Name 16-K104400-0022-924-D03C-BU45
GenBank Accession LN493418 [GenBank]
Sequencing primer gene specific primer, BU45: 5'-ATCTTCTTTGCTTCGTTTTGGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K104400-0022-924-D03C-BU45
TCAGGTCGTTTTTTTTGTTGTTGTGTAGGATTGTTGAGAGTGTTGGAGAAGGAGTGACTG
ATCTTCAGCCAGGAGATCATGTGTTGCCGATCTTTACCGGAGAATGTGGGGAGTGTCGTC
ATTGCCACTCGGAGGAATCAAACATGTGTGATCTTCTCAGGATCAACACCGAGCGAGGAG
GGATGATTCACGATGGTGAATCAAGATTCTCCATTAATGGCAAACCAATTTACCATTTCC
TTGGGACTTCCACGTTCAGTGAGTACACAGTGGTTCACTCTGGTCAGGTTGCTAAGATCA
ATCCGGATGCTCCTCTTGACAAGGTCTGTATTGTCAGTTGTGGTTTGTCTACTGGGTTAG
GAGCAACTTTGAATGTGGCTAAACCCAAGAAAGGTCAAAGTGTTGCCATTTTTGGTCTTG
GTGCTGTTGGTTTAGGCGCTGCAGAAGGTGCTAGAATCGCTGGTGCTTCTAGGATCATCG
GTGTTGATTTTAACTCTAAAAGATTCGACCAAGGTATTCAAAAAGATGATAGTCTGTTTT
TGACTATGTTCTTCTATAATCTCCCTTCACTTACATTGAATTTGATATGTTATTGGCCAT
ATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGC
   
Sequence Name 32-K104401-0022-924-D03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K104401-0022-924-D03C-8409
CTAAATTGACTGTTTATTGGTGGAGCGTACCGGAAGCTGTTCTACAGGACTTAAACCAAG
AACAAGTAATCGATTCTACACTGAAGATGAATGGACTCGCC

Last Updated on 06.12.2018 16:11